含脂食品辐照标志物烷基环丁酮检测技术研究进展-国际金融研究.PDF

第 34 卷 第 4 期 辐射研究与辐射工艺学报 J. Radiat. Res. Radiat. Process. Vol.34, No.4 2016 年 8 月 /fushe/CN/volumn/home.shtml August 2016 含脂食品辐照标志物 2- 烷基环丁酮检测技术研究进展 张海伟 张雨露 费 晨 周裔彬 （安徽农业大学茶与食品科技学院 合肥 230036 ） 摘要 对比分析欧盟标准 EN1785: 2003 方法，综述了近年来 2-烷基环丁酮(2-Alkylcyclobutanones, 2-ACBs)在 提取、净化与检测等方面的研究进展。采用乙腈溶剂直接萃取含脂辐照样品中的 2-ACBs→商品化固相萃取 小柱净化→GC-MS 检测是目前替代 EN1785 的潜力方法。固相微萃取、柱前衍生化及间接酶联免疫等方法具 有快速、节约溶剂、低检出限等优点，但需要对操作条件进一步优化。 关键词 2-烷基环丁酮，辐照，含脂食品，检测技术 中图分类号 TS205 ，TL99 DOI: 10.11889/j.1000-3436.2016.rrj.34.040102 Progress in studies on the detection technique of 2-alkylcyclobutanones in irradiated lipid-containing foods ZHANG Haiwei ZHANG Yulu FEI Chen ZHOU Yibin (School of Tea and Food Science, Anhui Agricultural University, Hefei 230036, China) ABSTRACT Recent advances in extraction, purification and detection methods of 2-alkylcyclobutanones (2-ACBs) were reviewed through comparing analysis of the EU standard (EN 1785: 2003). Using of direct extraction with acetonitrile, purification with commercial solid phase extraction columns and detection with GC-MS for 2-ACBs is probably the potential method which can be alternative of EN1785. The methods of SPME (Solid-phase microextraction), pre-column derivation and indirect ELISA (Indirect enzyme-linked immunosorbent assay) have many advantages including saving analyzing time and organic solvent, and lowering detection limit. However, the relevant operating conditions need to be further optimized. KEYWORDS 2-Alkylcyclobutanones, Irradiation,