南极海冰耐盐细菌NJ82的分子鉴定及其耐盐性初步研究-微生物学报.PDF

微生物学通报 APR 20, 2008, 35(4): 486~490 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 研究报告 南极海冰耐盐细菌NJ82 的分子鉴定及 其耐盐性初步研究 侯艳华 1 王全富 1, 2,3* 沈继红 3 缪锦来 3 李光友 3 (1. () 264209) (2. 222005) (3. 266061) 摘 要: 从 129 株南极海冰细菌筛选到 1 株耐盐细菌NJ82, 该菌最适生长盐度是 12%, 能够耐受 最高盐度为 18%。对该菌株进行 16S rRNA 基因序列的同源性和系统发育分析, 结果表明：菌株 NJ82 属于Pseudoalteromonas 属。从总蛋白质、脯氨酸、丙二醛(MDA)含量及膜透性变化等方面 对高盐的适应性进行初步探讨。结果表明, 当盐度介于3.3%~12.0 ％时, 随着盐度升高, 菌株的蛋 白质和脯氨酸含量呈快速增加趋势, 而 MDA 含量和膜透性变化幅度不大; 随着盐度进一步升高, 蛋白质含量呈下降趋势, 脯氨酸变化幅度不大; 而 MDA 含量升高和膜透性变化都达到极显著水 平(P0.01) 。这些重要生理参数的变化将有助于了解海冰细菌在高盐环境下的适应机制。 关键词: 耐盐性, 海冰细菌, 南极, 16S rRNA Molecular Identification of a Halotolerant Bacterium NJ82 from Antarctic Sea Ice and Preliminary Study on Its Salt Tolerance HOU Yan-Hua1 WANG Quan-Fu1, 2, 3 * SHEN Ji-Hong3 MIAO Jin-Lai3 LI Guang-You3 (1. School of the Ocean, Harbin Institute of Technology, Weihai 264209) (2. Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005) (3. Key Laboratory of Marine Bio-active Substances, First Institute of Oceanorgarphy, State Oceanic Administration, Qingdao 266061) Abstract: Halotolerant bacterium NJ82 was screened from 129 strains of Antarctic sea ice bacteria. Opti- mum salinity for the growth of this strain was 12% ( W/ V), and the highest salinity tolerance was 18% ( W/ V). The 16S rRNA gene sequences homology