考研资料：北京大学细胞生物学第六章基质与开内膜(中).pptVIP

Gated transport: Vesicular transport * Biogenesis of the Golgi apparatus in living parasites. a–h, Transgenic parasites stably expressing IMC1–CFP (blue) were transfected with plasmid DNA encoding GRASP–YFP (green). After 20 h of infection in HFFs, four parasites were imaged by time-lapse video fluorescence microscopy. Images were taken every 10 min for 7 h at 37 °C. Representative images at the indicated times are shown. Note that T. gondii. Replicates synchronously in a given vacuole, which permits simultaneous imaging of several cells at the same cell-cycle stage. i, j, Transgenic parasite