《高效纤维素降解菌的选育》-毕业论文.DOCVIP

武汉科技大学本科毕业论文 PAGE PAGE I 本科毕业论文 题目： 高效纤维素降解菌的选育 学 院: 化学工程与技术学院 专 业: 生物工程 学 号: 学生姓名: 指导教师: 日 期: 2011年6月 摘 要 产酶纤维素微生物在纤维素资源的再生转化，提高牲畜饲料利用率，堆肥的使用以及环境污染等方面具有很高的应用价值。而分离高效纤维素降解菌是这一内容的前提与基础。 本研究利用选择培养基CMC培养基，FPA摇床培养基，从广西香蕉秸秆的土壤，武汉武丰宰牛厂牛的瘤胃，武汉晨鸣造纸厂的污水排放口的污泥以及汉阳江边的土壤中分离得到6株降解纤维素的真菌。它们都具有较强的纤维素降解能力，尤其以编号为纸绿（从造纸厂污泥中筛选得到的绿色霉菌）和江白（江边土壤中筛选得到的无毛白菌）活性最高。随后对这6种菌株进行了形态学和生理生化鉴定，从中得到一株高酶活的菌株,其在CMC培养条件下酶活达到40U,在FPA培养条件下达到4.5U。 关键词： 纤维素降解菌； 筛选； 纤维素酶活； 鉴定  Abstract Microbial fermentation of cellulose micro-organisms, especially fungi in the conversion of cellulose into renewable resources and improve utilization of livestock feed, compost use and environmental pollution has a high application value. And efficient cellulose degradation bacteria can provide useful help in this regard. In this study, selective medium CMC medium, FPA shaker medium of banana stalk from the soil, Wuhan Department Store abundance of the rumen of cattle slaughtering plant, Wuhan Chenming paper mill sludge from the sewage outfall in the river and the Hanyang Street drag straw isolated six soil fungi degrade cellulose. They have a strong ability of cellulose degradation, particularly in the number of paper green (screened from the paper mill sludge green mold) and White River (river soil hairless white screened bacteria) the highest activity. And the 6 strains with a high activity were morphological, physiological and biochemical identification. Key words: Cellulose degrading bacteria; Filter; Cellulase activity; Identification. 目 录 TOC \o 1-3 \h \u HYPERLINK \l _Toc295227461 1 文献综述 PAGEREF _Toc295227461 \h 5 HYPERLINK \l _Toc295227462 1.1 纤维素概述 PAGEREF _Toc295227462 \h 5 HYPERLINK \l _Toc295227463 1.2 纤维素酶 PAGEREF _Toc295227463 \h 5 HYPERLINK \l _Toc295227464 1.2.1 纤维素酶的组成 PAGEREF _Toc295227464 \h 5 HYPERLINK \l _Toc295227465 1.2.2 纤维素酶的种类以及作用机理 PAGEREF _Toc295227465 \h 7 HYPERLINK \l _Toc295227466 1.3 纤维素分解菌类 PAGEREF _Toc295227466 \h 8 HYPERLINK \l _Toc2