分子生物学3sectioni3.pptxVIP

Libraries of DNA Molecules Can Be Created by Cloning;返回;I 1 Genomic libraries ;;Making a representative library ;Size of library (ensure enough clones);For example :for a probability of 0.99 with insert sizes of 20kb these values for the E. coli (4.6×106 bp) and human (3×109 bp) genomes are : N E.coli= =1.1 ×103 ;Genomic DNA libraries;To make a representative genomic libraries , genomic DNA must be purified and then broken randomly into fragments that are correct size for cloning into the chosen vector. ;Vectors ;; ;cDNA libraries;cDNA lib