整合素β1,FAK和pY397FAK在非小细胞肺癌组织中的表达.docVIP

整合素β1、FAK和pY397FAK在非小细胞肺癌组织中的表达 聂蓉 张红萍 武汉工业学院健康科学与护理系，湖北 武汉 430023 【摘要】目的：通过检测整合素β1亚基、FAK和其磷酸化形式pY397FAK在非小细胞肺癌（non-small cell lung cancer, NSCLC）和正常支气管粘膜上皮中的表达，探讨整合素β1与FAK在NSCLC发生、浸润和转移中的作用。方法：应用免疫组织化学方法检测整合素β1、FAK和pY397FAK在72例NSCLC、29例淋巴结转移癌和19例正常支气管粘膜上皮中的表达，并与临床病理指标结合起来进行分析。结果：FAK、pY397FAK在NSCLC中的表达（83.3%, 52.8%）显著高于正常支气管粘膜上皮（57.8%, 21.2%）(P＜0.05) ;在配对的原发癌（93.1%,68.9%）与淋巴结转移癌（100%，72.4%）中的表达差异无显著性（P＞0.05）。整合素β1在NSCLC（86.1%）与正常支气管粘膜上皮（68.4%）中的表达差异及其在配对的原发癌（82.2%）与淋巴结转移癌（93.1%）中的表达差异均无显著性（P＞0.05）。NSCLC中整合素β1表达与分化程度及TNM分期及淋巴结转移有关，FAK表达与分化程度、TNM分期及淋巴结转移有关，pY397FAK表达与TNM分期及淋巴结转移有关(P＜0.05)。整合素β1与FAK、pY397FAK在NSCLC中的表达无相关性（P＞0.05）。结论：整合素-FAK信号转导通路可能在NSCLC发生和发展过程中起重要作用。FAK可能作为判断NSCLC恶性转化、增殖及侵袭转移等行为的指标和治疗的靶点。 关键词：非小细胞肺癌；整合素；粘着斑激酶 中图分类号：734.2 文献标识码：A Significance and Expression of Integrinβ1, Focal Adhesion Kinase(FAK) and Phosphorylated Focal Adhesion Kinase(pY397FAK) in non-small cell lung cancer Nie Rong Department of Health Science an Nursing, Wuhan Polytechnic University, Wuhan, Hubei, 430023, China 【ABSTRACT】OBJECTIVE: To explore the roles of Integrinβ1, FAK and its phosphorylated form, i.e.pY397FAK, in tumorigenesis, invasion and metastasis of non-small cell lung cancer (NSCLC) by detecting the expression of Integrinβ1, FAK and pY397FAK in human normal bronchial epithelium, primary NSCLC and matched lymph node (LN) metastases. METHODS: Using immunohistochemical method, the expression of Integrinβ1, FAK and pY397FAK in 72 cancerous tissues and 29 LN metastases as well as 19 normal bronchial epithelia was measured. The relationship between Integrinβ1, FAK and pY397FAK expression and clinicopathological characteristics was analyzed. RESULTS: Elevated levels of FAK and pY397FAK expression were detected in primary tumor (85.5%, 56.4%) compared with normal lung epithelium (57.9%,21.1%)( P＜0.05). The expression of FAK and pY397FAK in matched primary tumors was not significantly lower than that in matched LN metastases(93.1% vs 100%, 68.9% vs 72.4%;P＞0.05 ). There was no