低温下冬小麦品种间甜菜碱及其代谢途径比较.PDFVIP

中国农学通报 2018 中国农学通报 2019,35(1):6-11 Chinese Agricultural Science Bulletin Chinese Agricultural Science Bulletin 低温下冬小麦品种间甜菜碱及其代谢途径比较 左 嵩 （哈尔滨市出入境检验检疫局食品处，哈尔滨150001） 摘 要：为研究甜菜碱以及其关键合成酶甜菜碱醛脱氢酶在低温胁迫时对小麦的保护作用，笔者将抗寒 性不同的2 个冬小麦品种‘东农冬麦一号’(DM1)和‘济麦22 ’(J22)栽培于自然条件下，通过测定苗期相 对电导率、丙二醛(MDA)含量、甜菜碱醛脱氢酶活性、甜菜碱醛脱氢酶基因表达量以及甜菜碱含量。结 果发现，抗寒品种DM 1表现出甜菜碱醛脱氢酶酶活和甜菜碱含量大幅增加，而封冻期MDA 含量相比 J22 却有所降低。这说明甜菜碱醛脱氢酶基因的表达促进了甜菜碱的积累，同时增加了细胞膜的稳定 性。本研究结果不仅确定了甜菜碱在DM 1抗寒性中的作用，同时为今后提高冬小麦抗寒性提供新的研 究方向。 关键词：冬小麦；抗寒；甜菜碱；甜菜碱醛脱氢酶 中图分类号：S332.1 文献标志码：A 论文编号：cas WinterWheatVarietiesUnderLowTemperature:GlycineBetaineandItsRelatedMetabolicPathways ZuoSong (FoodDepartment,HarbinEntry-exitInspectionandQuarantineBureau,Harbin150001) Abstract: The paper aims to study glycine betaine (GB) and the protection effect of betaine aldehyde dehydrogenase(BADH),whichisthe keysynthetase,onwheat.Theauthorplantedtwowinterwheatvarieties withdifferentcoldresistance, ‘Dongnongdongmai1’(DM1)and ‘Jimai22’(J22)undernaturalcondition,and analyzedtherelativeelectricalconductivity,malondialdehyde(MDA)content,BADHactivity,theexpression ofBADH,andthecontentofGBattheseedlingstage.Theresultsshowedthat:DM1presentedagreatincrease inBADHactivityandGBcontent,butadecreaseinMDAcontentunderfreezingconditioncomparedwithJ22, indicatingthattheexpressionof BADH promotedtheaccumulationof glycinebetaine andincreasedthe stabilityofcellmembrane.Theresultsofthisstudynotonlyconfirmtheroleof glycinebetaine incold resistanceofDM1,butalsoprovideanewresearchdirectionforimprovingthecoldresistanceofwinterwheat inthefuture. Keywords:winterwheat;coldresistance;glycinebetaine;BADH 0 引言 GB 。首先胆碱经过胆碱单加氧酶(CMO)催化形成甜 甜菜碱(GB) 的化学名称为N- 甲基代氨基