兔VX2肿瘤侵犯下腔静脉动物模型的建立和生物学.doc

1，[基金项目]重庆市卫生局医学科研项目面上项目（2010-2-041）2，作者简介：张元国，24岁，重庆医科大学附属第一医院血管外科在读硕士，联系方式： HYPERLINK mailto:cyzyg2009@163.com cyzyg2009@163.com．通信作者： 1，[基金项目]重庆市卫生局医学科研项目面上项目（2010-2-041） 2，作者简介：张元国，24岁，重庆医科大学附属第一医院血管外科在读硕士，联系方式： HYPERLINK mailto:cyzyg2009@163.com cyzyg2009@163.com． 通信作者：任为，男，42岁，重庆医科大学附属第一医院血管外科副教授，主要从事血管疾病基础与临床方面研究。联系电话联系方式： HYPERLINK mailto:renwei9771@ renwei9771@ 涉及多方面内容，不具体 张元国 任为 （重庆医科大学附属第一医院 血管外科，重庆 400016） 【摘要】目的：建立VX2肿瘤侵犯下腔静脉（IVC）的动物模型，并观察相应的生物学行为目的太大。方法：经腹直视下，将VX2肿瘤组织块分别种植在28只兔肾以下IVC附近的软组织内，然后随机分为A、B两组。A组术后每周在超声下观察肿瘤的大小，IVC和腹主动脉（AA）管径大小。B组每周随机解剖4只兔子，取IVC和AA送做病检，并观察转移情况。结果：（1），超声显示：所有肿瘤均种植成功，肿瘤面积大小与生长时间呈明显正相关（r1=0.879），IVC管径与肿瘤面积大小显著负相关（r2=-0.8295）；4周时25%的IVC管腔闭塞，5周时达到91.7%。；（2），病理检查：3周时IVC管壁中见大量肿瘤细胞，5周时AA管壁中未见肿瘤细胞；解剖观察：第4周开始出现肝、肺和腹腔转移。结论：VX2肿瘤成瘤率高，其生长变化、对IVC的侵犯和远处器官转移均随生长时间而变化明显。 目的太大 【关键词】下腔静脉；动物模型；VX2瘤；兔 中图分类号：R654.4 Observation of biological behavior in rabbits of the VX2 tumor invading infrarenal IVC Zhang Yuanguo Ren Wei （Department of Vascular Surgery ，First Affiliated Hospital of Chongqing Medical University； Chongqing ； 400016） 【Abstract:】Objective: To establish an experiment animal model of the VX2 tumor invading IVC and observe its biological behaviour.METHODS:Abdominal nasopharynx, the prepared VX2 tumor tissue mass was transplanted into the soft tissues around the infrarenal IVC in 28 rabbits, then the rabbits were randomly divided into group A and group B.Group A：The size of tumor, the diameter of the IVC and the AA were detected by ultrasound once a week as long as 5 weeks after implanted.GroupB: 4 rabbits were randomly dissected weekly, the IVC and the AA were to send for pathologic examination and metastasis in lung, liver, kidney and celiac was observed at the same time. RESULTS: 1, Ultrasound examination shows: all VX2 tumors had been growing successfully in the model rabbits, the tumors’ size were significantly positive related to time (r1=0.879，P0.001), the diameter of IVC were significantly