马氏珠母贝精子的超低温保存.PDFVIP

302 Vol.30, No. 2 2006 4 Apr., 2006 JOURNAL OFFISHERIES OF CHINA : 1000- 0615(2006)02- 0170- 05 王梅芳, 余祥勇, 刘 永, 毛 勇, 于非非 ( , 524025) : pH (5. 5~ 9.5), (,,) , ,1B10 1B20 ,4 , :( pH 7.5~ 8.0) ,10% DMSO , 1B20,(0~ 4 e )30 min,15 cm5 cm 5 min10 min (- 196 e ) ,24 h48 h 5 ,38~ 40 e , 0.25j ,60%,80% :; ; :S917 :A Study on cryopreservation of spermatozoa in Pinctada martensii WANG Me-i fang,YU Xiang-yong,LIU Yong,MAO Yong,YU Fe-i fei ( Fisheries College, Guangdong Ocean Univ ersity , Zhanj iang 524025, China) Abstract: Comparing solutions of different pH ( 5. 5- 9.5) and different salinity to develop suitable basic solution for spermatozoa cryopreservation of pearl oyster P inctada martensii , we chose the basic solution, which could inhibit the activation and had less influence on the physiological characteristics ( such as activity, life span and fertilizing capability) of the spermatozoa, and adopted dimethyl sulfoxide (DMSO) as antifreeze to compound diluentsfor protecting the spermatozoa. After combination of natural seawater with DMSO (10%) and seminal fluid at the ratios of 1B 10 and 1B20, the specimens were transferred into LN2 by 4 temperature- lowering processes. The conclusion showed that cryopreservation processes were effectivein whichthe sampleswerepre-frozen in0- 8 e within 30 minutes, swung at 15 cm above for 5 min and then at 5 cm above LN2 for 10min, transferred them into LN2 for prolonging period ( 24 h, 48 h and 5 months ) , and then thawed them with water at 35- 40 e , the survival rate of spermatozoa could exceed 60% when activated w