电离辐射对小鼠小肠绒毛上皮细胞和固有层纤维细胞作用的基因表达谱分析.pdfVIP

电离辐射对小鼠小肠绒毛上皮细胞和固有层纤维细胞作用的基因表达谱研究 英文摘要 The radiation effect on the gene expression spectrum of IECs and intestinal lamina propria fibroblasts of mouse Abstract Background: In the previous study of C60(OH)24-SLN-E for radiation preotection in mice, it has been found that the γ-H2AX foci in intestinal lamina propria were more than that in intestinal villus epithelial cells at the 21th day after 6 Gy 60Co  ray exposure. The experimental phenomena implied the difference of DNA repair potential between the two kinds of cell. Objective: To investigate the molecular mechanism of the different of DNA repair capability between the intestinal lamina propria and villus epithelial cells, and to find new molecular target for the treatment of intestinal radiation sickness. Methods: (1) The primary cells of IECs and intestinal lamina propria fibroblasts were cultured and subcultured in vitro. Each kind of cells divided into two groups, the experimental cell was irradiated by 6 Gy X-ray and the control cell was non-irradiated. (2) At 6 hours after irradiation, the γ-H2AX protein was evaluated by western blot assay. (3) At 6 hours after irradiation ，total RNA from each sample was isolated and the cDNA labelled with probe was hybridized to the mouse gene chip , after which the chip was washed and scanned, the gene expression differential spectrum was assessed by GO analysis and PATHWAY analysis . Results: (1) The primary cells of intestinal villus epithelial cells and lamina propria fibroblasts have been cultured successfully. (2) The γ-H2AX protein in the two kinds of cells were increased after X-ray irradiated and repaired six hours, however, there was no significant difference bet