甲氰菊酯降解菌HY1的降解特性及土壤修复.PDF

中国农学通报 2016,32(35): 111-116 Chinese Agricultural Science Bulletin 甲氰菊酯降解菌HY1的降解特性及土壤修复 侯晓娟，杨丽莉，侯志广，赵晓峰，张 浩 （吉林农业大学资源与环境学院，长春130118） 摘 要：研究甲氰菊酯降解菌HY 1 的降解特性及其对污染土壤的生物修复效果，为其实际应用奠定基 础。利用气相色谱法和摇瓶振荡培养法确定了HY 1 降解酶位置及类型，同时研究了底物浓度、接菌量、 pH 值、温度及土壤是否灭菌对降解效果的影响。研究结果表明，降解菌HY 1起降解作用的酶主要是胞 外酶且为诱导型酶；底物甲氰菊酯对HY 1 的降解活性起诱导作用。HY 1 降解甲氰菊酯的最优底物浓度 为10 mg/L ，最适条件为pH 7.0 ，最适HY 1接菌量应为6%（体积分数），最适降解温度为30℃。土壤修复 试验中甲氰菊酯最高降解率可达84.53% 。HY 1在未灭菌土壤中对甲氰菊酯降解速率比灭菌土壤快，说 明其能协同土著微生物共同降解甲氰菊酯。降解菌HY 1 能有效降解甲氰菊酯，并对甲氰菊酯污染的土 壤有较好的修复效果，为治理甲氰菊酯污染土壤提供了理论参考。 关键词：甲氰菊酯；降解菌；降解特性；土壤修复 中图分类号：X592 文献标志码：A 论文编号：cas DegradationCharacteristicsandSoilRemediationofFenpropathrinDegradingBacteriaHY1 HouXiaojuan,YangLili,HouZhiguang,ZhaoXiaofeng,ZhangHao (ResourceandEnvironmentCollege,JilinAgriculturalUniversity,Changchun130118) Abstract: The aim was to study the degradation characteristics and soil remediation of fenpropathrin degradingbacteriaHY1,inordertolayfoundationforitspracticalapplication.Bygaschromatographyand shakingthebottleshockculturemethod,thelocationandtypeofdegradingenzymeofstrainHY1 were determined.Atthesametime,theeffectsofconcentrationofsubstrate,theinoculumofHY1,pH,temperature andsterilizedsoilandunsterilizedsoilondegradationoffenpropathrinwerestudied.Theresultsshowedthat degradingenzymeofstrainHY1 wasextracellularenzymeandinducibleenzyme.Fenpropathrininducted degradationactivityofstrainHY1.Theoptimalconcentrationofsubstratewas 10mg/L,theoptimalpHvalue was 7.0,theoptimalinoculumofHY1was 6%(v/v),theoptimumdegradationtemperaturewas 30℃.HY1 repairedsoilthemaximumdegradationrateof84.53%intheexperimentsoilremediation.Thedegradationrate offenpropathrinbyHY1wasfasterinunsterilizedsoilthaninsterilizedsoil,whichindicatedthatitcould degrad