生物反应玉器课件bioreactorselection.ppt

* * * * * We recently integrated the SDR with our u-flask covers. This is now available as a catalogue item and gives researchers the ability to do hundreds of sensor containing shake flasks within a single incubator. This is the perfect intermediate for when users are already employing the u-flasks and need that next step before they go to the u-24 or bioreactors. * * Bioreactor selection ‘Scale-Up’ Bringing a Process to Scale Each product creates its own unique challenges for process development: Developing a Biofuels process to the 300,000L scale faces different challenges than developing a Pharmaceutical production process to the 100L-25,000L scale. Developing a Pharmaceutical production process in a microbial host faces different challenges than would be faced in a mammalian process. Despite the wide range of differences, most process development efforts require the same basic steps and these steps are a result of shared end goals. What is the Goal?... To run a multi-million (billion) dollar production facility at full capacity producing end products worth millions (billions) of dollars. How is this Goal Achieved? At the heart of the process is a stable, reliable cell line and culture approach which has been well characterized at a smaller and more manageable scale. Create Cell Line Select in Well-Plates Advance to Flasks, Select Further. Expand Cell Line Develop the Process Scale the Process Identify ProductRD Large Scale Process Identify ProductRD Selection Steps Development Steps Because these steps provide very little information to the researcher, “Selection” is possible, but true ‘process development’ is difficult. The data recorded at each step during process development is what allows the researcher to control and therefore scale the process. Information Available at Different Stages. Identify ProductRD Early, High-throughput stages provide little information. Researchers are often limited to Cell Counts and Titers. Process Development stages tr