低温诱导栽培番茄、多毛番茄和类番茄茄的转录组分析-生物学;植物学专业论文.pdf.docxVIP

Classified Classified Index： Code：10224 Confidential(yes／no)：no NO．：1209109 Dissertation for the Doctoral Degree The Study on Solanum lycopersicum， Solanum habrochaites，and Solanum lycoj aides underUnll LqOW TIlemDerature Base on Transcriptomes Candidate：Chen Hongyu Supervisor：Professor Wang Aoxue Degree Category：Doctor of Science College：Life Science First level discipline：Biology Second level discipline：Botony Harbin China June 2015 万方数据 目录 目录 ＼吣Y帆2㈣7 7㈣2㈣20㈣9 摘要 一 ．j-_：==： I 万方数据 东北农业大学理学博士学位论文3．5．3低温应答可变剪接基因的功能聚类分析 东北农业大学理学博士学位论文 3．5．3低温应答可变剪接基因的功能聚类分析 63 3．6栽培番茄和多毛番茄的SNP分析 65 3．7 MicroRNA识另0 ．65 3．7．1高通量测序 ．65 3．7．2新MicroRNA的识别 ．66 3．7．3 MicroRNA差异表达与低温应答MicroRNA的识别 一67 3．7．4 MicroRNA靶基因的识别 68 3．7．5低温应答MicroRNA．靶基因通路识别 一69 4讨论 ．．73 4。1低温应答基因识别 73 4．2低温应答可变剪接识别 76 4．3低温应答MicroRNA识别 77 5结论 ．．79 参考文献 一80 致{射 ．．85 攻读博士学位期间发表的学术论文 86 lI 万方数据 Co}ITENTSCONTENTS Co}ITENTS CONTENTS Abstract in(：hinese ．I Abstract in English ．．III 1 Introduction 1 1．1 Aims of research 1 1．2 Total document ． ．．1 1．2．1 Research progress of freeze—tolerant mechanism in plants ·1 1．2．2 Solanum habrochaites and Solanum lycopersicoides 4 1．2．3 Sequencing 5 2 Materials and Methods ．7 2．1 Materials 7 2．1．1 Plant materials ．7 2．1．2 Strain 7 2．1．3 Molecular biology reagents 7 2．1．4 Culture medium ．．7 2．1．5 Equipments 8 2．2 Methods ． ．．．． ． ．．． ． ． ．．．．． ． ． ．8 2．2．1 Physiology and biochemical indicator ．．8 2．2．2 RNA sequencing and data analysis ．9 2．2．3 Q．PCR ． ．10 3 Results analysis ．．12 3．1 Phenotypic and physiological 12 3．2 Transcriptome analyses 14 3．2．1 RNA sequencing ． ．14 3．2．2 Mapping ． ．16 3．3 Differential expression genes and GO enrichment ．．1 8 3．2．1 Analysis ofthe sample correlation ．．18 3．2．2 Q．PCR ． ． 21 3．2．3 Differential expression genes ．． ．．26 3．4 GO analyses of cold．regulated gene ．．34 3．4．1 GO analyses ofcold．regulated gene in tomato and Solanum habrochaites ．．38 3．4．2 GO analyses of cold．regulated gene in Solanum lycopersicoides ．50 3．4．3 Cold．regulated genes involved