《金鱼脂肪酸结合蛋白7的克隆与表达分析论文》-毕业论文设计（可用）.doc

河南理工大学本科生毕业论文 摘要 PAGE I 本科毕业设计（论文） 题目 金鱼脂肪酸结合蛋白7的克隆与分析 院 (系) 资源环境学院 专业名称 生物技术 年级班级 2007级01班 学生姓名 张瑞 指导老师 陈文波 二零一一年六月十日 摘 要 本文研究的脑型脂肪酸结合蛋白（Brain fatty acid-binding protein）属于脂肪酸结合蛋白家族成员之一。我们通过使用生物信息学分析以及实验的手段，对金鱼脑型脂肪酸结合蛋白（B-FABP或FABP7）的基因序列及氨基酸序列特征进行了研究。选去了7种不同生物个体的脑型脂肪酸结合蛋白进行了同源性比对、进化树分析，并对金鱼脑型脂肪酸结合蛋白进行了肽链信号肽、O糖基化、N糖基化位点的预测。并且提取了金鱼8种组织中的总RNA，经过RT-PCR、琼脂糖凝胶电泳等手段对其进行了B-FABP的组织特异性分布的研究。 预测结果显示其与斑马鱼的同源性最高可达90%以上，组织特异性分布只在金鱼的脑组织有表达。这些结果为进一步研究金鱼的脑型脂肪酸结合蛋白提供了可靠地参考依据。 关键词：金鱼（Crassus aurous）；脑型脂肪酸结合蛋白；生物信息学分析；克隆；组织表达 河南理工大学本科生毕业论文 Abstract II Abstract In this paper, we researched the brain-type fatty acid binding protein is a member of the fatty acid binding protein family. We used some means of bioinformatics and experiment to study goldfish brain fatty acid-binding protein gene sequence and amino acid sequence features, and selected the 7- brain fatty acid-binding protein gene sequence from different species and contrasts with homologous relationships, evolutionary tree, signal peptide and O glycosylation, N glycosylation sites prediction. And extracted 8 tissues total RNA, by RT-PCR, agarose gel electrophoresis to carry out B-FABP in tissue-specific. Predicted results showed that the brain fatty acid-binding protein of zebrafish A with the highest homology and up to more than 90%. And the B-FABP mRNA was expressed only in goldfish brain tissue. It suggests that its expression is tissue-specific. These results provide a reliable reference for the further study of goldfish brain type fatty acid binding protein. Keywords: Goldfish (Crassus aurous); Brain fatty acid-binding pr