嗜酸产甲烷菌及其在厌氧处理中的应用.PDF

应 用 生 态 学 报摇 2011年2月摇 第22卷摇 第2期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 ChineseJournal of Applied Ecology,Feb. 2011,22(2):537-542 嗜酸产甲烷菌及其在厌氧处理中的应用* ** 郭晓慧摇 吴伟祥 摇 韩志英摇 石德智 (浙江大学环境与资源学院环境工程系,杭州310029) 摘摇 要摇 产甲烷菌在自然界碳素循环过程中发挥着重要作用.酸性泥炭沼泽环境中存在着多 种未知的产甲烷古菌,其中嗜酸产甲烷菌因其特殊的生长代谢特征近年来引起学者的广泛关 注.若将嗜酸产甲烷菌应用于高浓度有机废物或废水的厌氧消化过程中,可从本质上克服因 酸积累造成的产甲烷抑制,减少运行成本,扩展厌氧消化处理技术的应用范围.本文综述了嗜 酸产甲烷菌的富集分离培养方法、生理生化特性、代谢特征及相关分子生物学研究等内容,并 对其在厌氧处理中的应用前景进行了分析和展望,提出了未来研究的方向. 关键词摇 酸积累摇 嗜酸产甲烷菌摇 生理生化特性摇 厌氧处理 文章编号摇 1001-9332(2011)02-0537-06摇 中图分类号摇 Q142.9;X172摇 文献标识码摇 A Acidophilic methanogens and their applications in anaerobic digestion. GUO Xiao鄄hui,WU Wei鄄xiang,HAN Zhi鄄ying,SHI De鄄zhi (Department of EnvironmentEngineering,College of Envi鄄 ronment and Resource Sciences,Zhejiang University,Hangzhou 310029, China).鄄Chin. J.Appl. Ecol. ,2011,22(2):537-542. Abstract:Methanogens play an important role in global carbon cycle. There exists a range of un鄄 known methanogenic archaea in acidic peat lands,amongwhich,acidophilic methanogenshave at鄄 tracted increasing research interests because of their special metabolic characteristics. To introduce acidophilic methanogens in the anaerobic digestion process of high concentration organic wastes or waste water could essentially overcome the inhibition of acid accumulation on the methanogens and help reduce the operation cost, broadening the industrial application of anaerobic bio鄄treatment technology. In thispaper,we reviewed the recent researches on acidophilic methanogens,with the focus on enrichment and isolation methods, physiological and biochemical characters, metabolic characteristics,andapplicationof molecularbiology. Thepotentia