5HT1A受体对新生大鼠离体延髓脑片呼吸节律性放电调制.pdf

生理学报 Acta Physiologica Sinica, June 25, 2007, 59 (3): 293-298 293 研究论文 5-HT1A 受体对新生大鼠离体延髓脑片呼吸节律性放电的调制 * 秦峥，吴中海 ，王晓锋 南方医科大学生理学教研室，广州 510515 摘 要：本研究探讨5-HT 1A 受体在延髓基本节律性呼吸放电发生和调节中的作用。以改良Kreb’s 液恒温灌流新生Sprague- Dawley 大鼠离体延髓脑片标本，稳定记录与之相连的舌下神经根的呼吸节律性放电活动(respiratory rhythmical discharge activity, RRDA)后，第一组在灌流液中分别单独给予5-HT 1A 受体的特异性激动剂8- 羟四氢萘[(+/-)-8-hydroxy-2-(di-N - propylamino)tetralin hydrobromide, 8-OHDPAT]和特异性拮抗剂多次甲基多苯基多异氰酸酯[4-iodo-N -[2-[4-methoxyphenyl]- 1-piperazinyl]ethyl]-N -2-pyridynyl-benzamide hydrochloride, PMPPI] ；第二组分别先后给予8-OHDPAT 和8-OHDPAT + PMPPI ，观察舌下神经根RRDA 的变化，探讨5-HT 1A 受体对其调节作用。结果显示，给予8-OHDPAT 后，呼吸周期 (respiratory cycle, RC)和呼气时程(expiratory time, TE)显著延长，放电的积分幅度(integral amplitude, IA)持续降低，吸气时 程(inspiratory time, TI)无明显变化；给予PMPPI 后RC 、TI 和TE 明显缩短，舌下神经根IA 无明显变化，且8-OHDPAT 的作用可部分被PMPPI 逆转。结果提示，5-HT 1A 受体参与了哺乳动物基本呼吸节律的产生和调节。 关键词：5 - H T 1 A 受体；延髓：呼吸节律；节律性放电 中图分类号：Q 4 2 ；R 3 38 . 2 5-HT1A receptors are involved in the modulation of respiratory rhythmical discharge activity in the medulla oblongata slice preparation of neonatal rats * QIN Zheng, WU Zhong-Hai , WANG Xiao-Feng Department of Physiology, Southern Medical University, Guangzhou 510515, China Abstract: The present study was carried out to determine the role of 5-HT1A receptors in the generation and modulation of basic respiratory rhythm. Neonatal (aged 0-3 d) Sprague-Dawley rats of either sex were used. The medulla oblongata slice was prepared and the surgical procedure was performed in the modified Kreb’s solution (MKS) with continuous carbogen (95% O and 5% CO ), and ended in 3 min. 2 2 In cold MKS, a 600-700 μm single transverse slice was cut, which was rostral to the edge of area postrema and retained the hypoglossal nerv