b超引导下对犬重要器官的活组织穿刺检查初探兽医专业论文.docxVIP

簧式半自动活检穿刺枪，在最佳穿刺位点成功采集了肝、脾、肾组织样本， 簧式半自动活检穿刺枪，在最佳穿刺位点成功采集了肝、脾、肾组织样本， 通过病理组织学检查确认，上述组织样品具有完整肝、脾、肾的组织结构。 肝脏切片可以清晰观察到门管区和肝小叶，但是犬的肝小叶间结缔组织不 发达，所以分界不清；肝细胞核大而圆，肝小叶中肝细胞以中央静脉为中 心，向周围呈放射状排列，肝索整齐，肝血窦明显；门管区内有小叶间静 脉，管腔大而不规则；小叶问动脉管腔小而壁厚；小叶间胆管壁单层立方 上皮排列整齐。脾脏切片可以明显区别脾脏的白髓、红髓、边缘区，红髓 中髓静脉管腔较大，横截面清晰可见，且部分红髓处于充血状态。肾脏切 片可清楚观察到肾皮质的肾小体和肾小管，肾小体成球形，肾小体中有一 团弯曲的毛细血管，肾小体外围有两层囊壁形成肾小囊腔，与肾小管近曲 小管相通；近曲小管管径粗，管腔小且不规则，管壁上皮细胞细胞界限不 清，腔面有刷状缘；远曲小管管腔大而规则，管壁上皮细胞为立方形，腔 面无刷状缘。 结论：本试验通过B超引导在犬身上运用细针抽吸和弹簧式半自动活 检枪，成功经皮穿刺取出少许肾脏、肝脏、脾脏组织和胆汁，并进行病理 学检查获得预期结果，操作方法无潜在并发症，且恢复良好。进一步确定 了这个微创诊断手段是可行的、安全的。 关键词：B超活检细针抽吸肝脏脾脏肾脏 Il 万方数据 E船LOR E船LOR ATl0N OF PUNCH BIOPSY FOR CANINE V11：AL ORAGANS 1■哏OUGH B．MODE AB STRACT A ultrasound-guided punch biopsy for internal organs(kidney,liver,spleen in this case)，is generally an minor traumatic examination for pathology analyze by using biopsy needle to extract certain number of splanchnic tissue．The biopsy samples have its diagnostic value and making great contribution to build the relation between clinical symptom and pathomorphism．Furthermore，the pathological mechanism provides direction for future treatment and prevention of focal lesions．Compared谢tll other kinds of biopsy methods，the 1’1 ultrasotmd-guIdea 1910psy teclamque not only otters a better pertbrmance on surgical accuracy but also has more advantages with simpliness and safety．In China，the application of biopsy through ultrasonography in small animal clinical practice lags behind．Here trials of biopsy for canine kidney,liver and spleen ultrasound-guided were carried out with fine-needle aspiration and biopsy needle to confirm its application possibility． After general anesthesia，ultrasonography was carried on dogs for determination of the best aspiration location，combining color doppler flow image in order to protect macro-vascular from injuries．The fine needle aspiration implemented for cytological examination．After making small incision III 万方数据 in in the skin，punch biopsy was done using a biopsy needle．The wax s