反义RNA技术在食品工业中的应用.doc

. 页 云南农业大学 反义RNA技术在食品工业中的应用 姓名：李晓莉 专业：食品质量与安全 班级：2012级食品质量安全1班 学号：2012310093 指导老师：冯励 日期：2014年10月8日 反义RNA技术在食品工业中的应用 摘要：随着反义RNA的发现及对其研究的深入,反义RNA技术已被广泛应用于食品工业的研究中。本文介绍了反义RNA的概念,并就反义RNA的作用机理和在食品工业领域的应用进行了综述。反义RNA最初在原核细胞中被发现并广泛存在于多类生物中，通过与mRNA互补结合形成杂交链而抑制基因的表达，作用于DNA的复制、转录、翻译的过程，实现对基因表达的调控。反义技术是最近20年来发展的一门全新的基因工程技术, 它是从反向遗传学的角度来认识结构基因的功能和基因表达的调控。反义RNA技术是借助基因重组技术根据碱基互补原理用人工合成或生物体合成的特定RNA片段或其化学修饰物抑制或封闭基因表达的技术。目前人们利用反义RNA技术的这一特点，在食品工业这一领域对生物基因进行有效调控并取得了显著的成就，并且这项技术具有极其广泛的应用前景，也将开辟一个新的领域。 关键词：反义RNA技术 基因工程 食品工业 应用 Antisense RNA technology application in food industry Industry With the discovery of antisense RNA and the deepening of research, antisense RNA technology has been widely used in the food industry research This paper introduces the concept of antisense RNA and antisense RNA mechanism and applications in food industry are reviewed.Abstract:Antisense RNA was originally discovered in prokaryotic cells and widely exists in many biological, combined with complementary mRNA hybridization to form chain and inhibit the expression of genes, role in the process of DNA replication, transcription, translation, realize the regulation of gene expression. Antisense technology is developed in recent 20 years of a new genetic engineering technology, it is from the perspective of reverse genetics knowledge structure gene function and gene expression regulation. Antisense RNA technology is with the aid of genetic recombination according to the principle of complementary base with synthetic or synthetic organism specific RNA or inhibit or closed its chemical membranes.It technology of gene expression. There are the characteristics of antisense RNA technology, in the field of biological genetic food industry for effective control and has made remarkable achievements, and this technology has the extremely widespread application prospect, will also open up a new field. Key words：Abstract:Antisense RNA genetic engineering food industry application 随着基因工程技术的蓬勃发展和代谢调控研究的深入, 反义技术作为一种温和调控