转Cry1AcCpTI双价抗虫水稻外源基因逃逸后在野生稻中-BioPublisher.docVIP

PAGE PAGE 1 转Cry1Ac/CpTI抗虫基因水稻外源基因在野生稻传代中的遗传和表达? 基金项目： ? 基金项目：国家转基因重大专项：转基因水稻环境安全评价技术(2008ZX08011-001) 苏军? 张高阳 余文杰*通讯作者： sj@+ 现工作单位：漳州职业技术学院食品与生物工程系 *通讯作者： sj@ + 现工作单位：漳州职业技术学院食品与生物工程系 363000 (福建省农业遗传工程重点实验室 福建省农业科学院生物技术研究所 福州 350003） 摘要：随着转基因水稻商品化进程，转基因水稻大面积释放后，外源基因逃逸到野生近源种，可能带来生态效应愈来愈受到人们的关注。本研究采用人工杂交技术获得Cry1Ac/sck双抗虫基因普通野生稻F1及分离后代F2、F3、F4、F5和回交后代BC1F1、BC1F2，研究转基因水稻外源基因逃逸后在普通野生稻（Oryza rufipogon Griff）传代中遗传和表达。结果显示：传代中外源基因的拷贝数与转基因亲本一致，外源基因插入位点侧翼序列扩增及其产物测序结果显示：外源基因在野生稻传代过程中插入位点的遗传也是稳定的。 Cry1Ac 关键词：转Cry1Ac/CpTI基因水稻；野生稻；遗传； 表达 Inheritance and Expression of Exogenous Genes in Wild Rice Descendants Su Jun Zhang Gao Yang Yu Wen Jie Song Hui Fujian Provincal Key Laboratry of Genetic Engineering For Agriculture. Biotechnology Institute Fujian Academy of Agricultural Sciences ,Fuzhou 350003，China Abstract：The extensive enviromental release and cultivation of GM crop varieties have aroused tremendous biosafty concerns and debates worldwide. One of the debates issue is transgene escape to wild relative species through gene flow and its potential ecological consequence. In this present we introduce transgene to wild rice by crossing with a transgene rice which carry 2 insect resistent genes Cry1Ac/sck and investigated the inheritance and expression of transgene in wild rice and its desendants. Molecular hybridization indicated that the cry1Ac gene were stably transmitted in an intact manner via successive sexual generations, the flanking sequences of insertion site were also stably transmitted in hybrid and its descendants. The concentration of the Cry1Ac protein were kept quantitatively stable up to the F5 generation. Our data indicated that the foreign genes are stable inheritance and expression Keywords: Cry1Ac/CpTI transgenic rice; Oryza rufipogon Griff ; Heredity; Expression 近年来，转基因作物商品化种植面积迅速扩大，2009年全球转基因作物种植面积已经达到1.34亿公顷（/）。水稻是亚洲及中国最重要的粮食作物之一，水稻也是虫害较为严重的作物(韩兰芝等2006)，每年因虫害造成的经济损失达400-500万t（王艳清，2006）。应用植物基因工程技术，培育抗虫转基因水稻是解决水稻虫害问题的根本手段。2009年我国批准了2份抗虫转基因水