临床医学毕业血管内皮生长因子对骨髓间充质干细胞向软骨细胞分化的影响.docVIP

XX大学 毕业论文 血管内皮生长因子对骨髓间充质干细胞向软骨细胞分化的影 咆 姓 名： 2014年6月25日 血管内皮生长因子对骨髓间充质干细胞向软骨细胞分化的影响 作者：王开友，张鲁平，王颖，潘琪 【摘要】 ［目的］探讨外源性血管内皮生长因子(VEGF)对人骨髓来源间 充质干细胞(MSCs)增殖、向成软骨方向定向分化等生物学行为的影响及其机 理，为MSCs构建组织工程化软骨奠定基础.［方法］取人骨髓来源间充质干细 胞体外培养,将传代后的细胞分别置于含有800 ng / ml和1 600 ng / ml VEGF 的诱导培养基中进行培养，将具有促进细胞增殖分化及抑制多种炎性介质活性等 多重生物学效应的TGF pl以10 ng/ml浓度配置无血清培养基诱导同组MSCs 作阳性对照，观察细胞的增殖，分化。用II型胶原免疫组化染色评价不同诱导因 素下的软骨基质合成情况。［结果］所有细胞显示了良好的增殖能力，暴露于外 源性血管内皮生长因子下的MSCs保持了良好的生长活性，并开始向软骨表型分 化，与未添加诱导因子组细胞相比差异有显著性意义，不同浓度VEGF组的细 胞合成软骨基质的能力无差异，但均不如阳性对照组。［结论］外源性VEGF具 有诱导体外培养的人MSCs向软骨表型分化的能力，但与TGF pl的诱导能力 相比有差距。提示VEGF在MSCs来源的软骨修复过程中充当诱导信息提供者 的角色。 【关键词】 间充质干细胞；血管内皮生长因子；软骨细胞；分化 Abstract： ［Objective］ To study the effect of vascular endothelial growth factor(VEGF)on proliferation of human bone marrow derived mesenchymal stem cells(h MSCs)and its chondrogenic differenciation and to provide a basis for the tissue engineering technology of articular cartilage?［Method］ The hMSCs were cultured in vitro as usual and its passage was induced the by medium containing 800 ng/ ml or 1600 ng/ ml the VEGF MSCs the cultured in the medium containing 10 ng/ ml the TGF p 1 which the had multiple the biological efficacies the to enhance the proliferation the of MSCs the and to the inhibit the activities of the inflammatory factors,acted as a positive control.The induced MSCs were used to observe its proliferation and diflerenciation.The synthesis of chondrocyte extracellular matrix in different induce factor was measured by immunohisto chemical staining. [Result ] MSCs cultured wit different induced factors proliferated well.MSCs induced by VEGF medium showed a well growth activity and chondrogenic differenciation,but MSCs cultured in TGFpl containing medium induced more chondrocyte matrix than that in VEGF medium?[Conclusion ] VEGF induces chondrogenic differentiation of MSCs in vitro,but MSCs cultured in TGF卩 1 medium induces more chondrocyte matrix.lt is suggested that VEGF acts as donor of inducer message cartilage repair by MSCs. Key