碧流河水库底泥氨氧化微生物丰度及群落研究环境工程专业论文.docxVIP

碧流河水库底泥氨氧化微生物丰度及群落研究 Research on Community and Abundance of Ammonia．oxidizing Microorganisms in Sediment of Biliuhe Reservoir Abstract Ammonia oxidation microorganisms，mainly including ammonia oxidizing bacteria and ammonla oxidizing archaea,play a key role in the process of ammonia oxidation．Meanwhile ammonia oxidation process is the limiting step in the process of nitrification．So to study the diversity and abundance of ammonia oxidation microorganisms has a crucial role in understanding the ability of nitrogen metabolism and ecological stability，and it is also helpful to understand the whole nitrogen cycle．Biliuhe reservoir iS an important water source in Dalian．Due to serious soil and water loss，nitrogen，phosphorus，potassium，pesticides and other harmful substances flow into the reservoir every year，which haS the potential to cause the eutrophication and pollution in water body，and even destroy the balance of the nitrogen cycle in the reservoir．In order to reduce the speed of eutrophication in Biliuhe reservoir and maintain ecological stability，this experiment mainly studied the abundance and diversity of AOA、AOB in sediments of Biliuhe reservoir and the influence of environmental factors On the ammonia oxidation microorganisms． Sediments were sampled from ten different sites in the upstream．midstream and downstream of the reservoir in two different periods(1evel period in October 20 1 4。dry season in April 20 1 5)．This experiment studied the abundance of the specific amoA gene in ammonia oxidation mlcroorganlsms by reaI time quantitative Polymerase Chain Reaction(RT．PCRl， and analyzed the diversity of the gene by cloning sequencing．The result indicated that the copy numbers of AOA amoA gene in sediments were between 2．1 2 1 0 7 copies／g dry sediment and 8．92 1 06 copies／g dry sediment in October 20 1 4 while they were between 3．58x10 copies／g dry sediment and 1．0l×108copies／g dry sediment in April 2015．The abundance of AOA amoA genes in sediments did not chang