青霉菌、放线菌株和石灰水对尖孢镰刀菌抑制作用的研究.PDFVIP

中国农学通报 2013,29(12): 185-189 Chinese Agricultural Science Bulletin 青霉菌、放线菌株和石灰水对尖孢镰刀菌抑制作用的研究 王 芳，李 静，张 欢 （佛山科学技术学院，广东佛山528000） 摘 要：本研究旨在开发对土壤病原菌尖孢镰刀菌具有拮抗作用的各种微生物，以期对其生防制剂的研 制提供理论依据。利用PDA 平板对峙法和制备发酵菌原液，以青霉菌、放线菌株和石灰水对尖孢镰刀 菌孢子萌发、营养竞争、菌丝生长和重寄生作用进行了研究。结果表明：青霉菌、放线菌株和饱和石灰水 对尖孢镰刀菌的菌丝生长有不同程度的抑制作用，拮抗距离分别达到27.35、25.70、16.83 mm ，菌丝生长 抑制率分别为78.36%、75.97%、47.84% ，孢子萌发抑制率分别为71.97%、58.23%、21.49% 。在青霉菌、放 线菌株与尖孢镰刀菌的交接处没有发现明显的重寄生现象，青霉菌孢子大量繁殖，占据了大部分的生存 空间。青霉菌对尖孢镰刀菌在营养竞争、抗生作用方面均有较明显的作用，具有较好的抑制作用，可作 为拮抗菌进一步研究。 关键词：尖孢镰刀菌；菌丝生长；孢子萌发；抑制作用；青霉菌 中图分类号：S432 文献标志码：A 论文编号：2012-2909 The Study on Inhibition of Penicillium, Actinomycete and Limewater to Fusarium oxysporum Wang Fang, Li Jing, Zhang Huan (FoshanUniversityofScienceandTechnology, Foshan Guangdong 528000) Abstract: The aim was to study the various antagonistic microorganisms to Fusarium oxy sporum. in order to provide a theoretical basis in development of biological preparation. By PDA flat confrontation method and fermentation broth, the competition, hyperparasitism, antibioticaction of penicillium, actinomycete and limewater on Fusarium oxy sporum were studied. The result showed: penicillium, actinomycete and limewater had the different Inhibition on the mycelium growth of F. oxy sporum, the distance of antagonism were respectively 27.35 mm, 25.70 mm and 16.83 mm, the inhibited rate were 78.36%,75.97% and 47.84%, the inhibited rate of spore germination were respectively 71.97% , 58.23% and 21.49% , in the intersection of penicillium, actinomycete and limewater between Fusarium oxy sporum had no obvious hyperparasitism, the conidium of penicillium multiplicated numerously, occupied majority of the space. Penicillium