宫颈癌患者外周血淋巴细胞亚群分布的差异分析.doc

宫颈癌患者外周血淋巴细胞亚群分布的差异分析 ［摘要］目的检测分析宫颈癌患者外周血淋巴细胞亚群的 差异分布及临床意义。方法 采用流式细胞术检测宫颈癌患者和 健康对照成人外周血中CD3+、CD3+CD4+、CD3+CD8+、NK、NKT、 CD8+CD28+. CD8+CD28-、 CD3+HLA-DR+、 CD3-HLA-DR+、 Treg 细 胞的百分比，同时结合宫颈癌患者的临床诊断分期做对比分析。 结果 与健康对照组比较，宫颈癌患者外周血中CD3+（P二0. 0002） 和 CD3+CD4+ T 细胞百分比(P=0? 0004)以及 CD3+CD4+/CD3+CD8+ 比值（P二0.0256）显著降低；宫颈癌患者CD3+CD8+ T细胞百分 比与对照组比较无显著差异，但是随着患者临床分期的提高呈降 低趋势（P二0.0480 ）。NK细胞（P〈0?0001 ）、活化T细胞 (CD3+HLA-DR+: P0. 0001 ) 活化 B 细胞 (CD3-HLA-DR+: P二0.0021)百分比显著提高；细胞毒性T细胞(CD8+CD28+)细 胞百分比降低（P二0.0006）,抑制性T细胞（CD8+CD28-）百分比 提高（P二0.0009）； CD4+CD25brightT 细胞（Treg）阳性细胞百 分比与对照组比较没有显著差异，但CD4+CD25brightT细胞 （Treg ）在CD4+淋巴细胞中的百分比显著高于对照组 （P二0.0335）。宫颈癌患者外周血中CD8+CD28-/CD8+CD28+之比 与对照组比较显著提高（P0. 0001）o结论 宫颈癌患者细胞免 疫功能降低。精确分析宫颈癌患者的T淋巴细胞亚群变化对于临 床诊断、治疗和预后评估具有重要的实际意义。 ［关键词］宫颈恶性肿瘤；淋巴细胞亚群；流式细胞术；肿 瘤免疫 ［中图分类号］R737. 3 ［文献标识码］A ［文章编号］ 1673-9701 (2016) 24-0020-05 Analysis of the differences of distribution of lymphocyte subsets in peripheral blood in the patients with cervical cancer YU Hongl ZHANG Gengyuel JIN Yulongl DI Nal HE Chunyingl WEI Tianxuel DU Renqiu2 1. Cell Biology Research Office, Jilin Research Center for Tumor Prevention and Control, Changchun 130012, China； 2.Disease Record Room, Jilin Tumor Hospital, Changchun 130012, China [Abstract] Objective To test and analyze the differences of distribution of lymphocyte subsets in peripheral blood in the patients with cervical cancer and its clinical significance? Methods Percentages of CD3+, CD3+CD4+, CD3+CD8+, NK, NKT, CD8+CD28+, CD8+CD28-, CD3+HLA-DR+, CD3-HLA-DR+, Treg cells were tested in adult peripheral blood in the patients with cervical cancer and health controls via flow cytometry. Comparative analysis was conducted in combination with the clinical diagnosis staging in the patients with cervical cancer? ResuIts Compared with the healthy control group, the perce nt ages of CD3+ (P二0.0002) and CD3+CD4+ T cells (P二0?0004) in the peripheral blood in the patients with cervical cancer and CD3+CD4+/CD3+CD8+ ratio (P二0. 0256) were signi.ficantly