Target mRNA abundance dilutes microRNA and siRNA activiy靶mRNA丰度的microRNA和siRNA活性稀释.pptVIP

Target mRNA abundance dilutes microRNA and siRNA activity;Concept: Small RNAs with many targets downregulate each individual target to a lesser extent;microRNAs induce different amounts of downregulation;Meta-analysis of transfection studies;Off-Target Concentration;Pairwise examples;Kinetics Questions;Velocity is correlated with target abundance and follows Michaelis-Menten kinetics;ERIK’S SLIDE;Recent Literature;Questions;Pairwise examples;Consequences;Consequences;;Recent Literature;Background: RISC Kinetics;Haley Zamore (2004);Past Evidence: Dilution In Solution ;We control for several