番茄抗多种病害及耐贮种质资源的筛选园艺学蔬菜学专业论文.docxVIP

Cohrn孙rrSCoNTENTS Cohrn孙rrS CoNTENTS Abstract ill Chinese ．J Abstract in English ．．．．．． ． ．． ． ．．． ．．．．． ．．．．．．．．． ．．．．．．．． ．．．．．．．．．． ．．．．．．．．Ill I Introduction．．．．．．．．．．．．．．．．．．．．．． ．．．．．．． ．．．．． ．．． ．．．． ．．．．．．．．． ．．．． ．．．．． ． ．．．．．．I 1．1 Proppose and Meaning ofThis Study ． 一 1 1．2 Research ．。．．．．．． 。．。．．．．．． 。。．． ． ．．．．．。 。．。．。． 。。。。 ．。． ．．．．． ．。．．。．．。．． ．．。．．．．．．．1 1．2．1 Research progress tomato tobacco mosaic virus disease resistance breeding ．．1 1．2．2 Research progress tomato root-knot nematode disease resistance breeding ．．．．．2 1．2．3 Research progress tomato yellow leafcurl disease resistance breeding． ．．．．．．．．．．3 1．2．4 Research progress tomato Fusarium wilt disease resistance breeding． ． ．．．．．．．．4 1．2．5 Research progress tomato late blight disease resistance breeding．．．．．．．．． ．．．．．．．．．．5 1．2．6 Research progress tomato long shelf life breeding 6 1．2．7 Research progress tomato Flavor quality ． ． 7 1．3 Development and Application ofMolecular Marker．．．．．．．．．．．．． ． ．．．．．．．．．． ．．．． ．．．．．．．．．．．．．．．．．9 1．3．1 Application ofmolecular markers in breeding ofvegetables ．9 1．3．2 Molecular design breeding．．．．． ． ． ．． ．．．． ．．．．．．．．． ．．．．． ．．．．．．．．．．．10 2 Materials and Methods ． ． 1：1 2．1 Experimental Materials ． ．12 2．1．1 Experimental tomato materials ． ．12 2．1．2 Materials and sources ofPathogens．．．．．．．．．．．． ．．． ．．．．．．．．．．． ．．． ． ．．．．．．．．．．．．．．．．．．．12 2．1．3 Primers source ．．12 2．2 Apparatus and Reagents oftest．．．．．。。。。 。．。。 。 。 ．． ．．．．。．．． 。．．．。．。 。．。．．．．．，。．．．．．。。．．，，。13 2．3 Test Method ．1 3 2．3．1 Construction of six generation population． ．． ．． ． ．．．．．．．．．． ．．．．．1 3 2．3．2 Culture and inoculated identification oftobacco mosaic virus 14 2．3．3 Culture and inoculated identification of Meioidogyne incognita 一1 5 2．3．