废水处理系统中降解基因的克隆新技术研究及多样性分析植物学专业论文.docxVIP

兰州大学硕士学位论文 兰州大学硕士学位论文 废水处理系统中降解基因的克隆新技术研究及多样性分析 作中提供了借鉴，用于克隆的宿主E．coli菌株的选择将是一个重要的考虑因素。另外 一类克隆中具有启动子活性的基因片段，但克隆获得的启动子为未知序列，还需后 续的深入研究。 关键词：杂环芳香化合物，功能基因，降解，启动子捕获 IlI 兰州大学硕上学位论文 兰州大学硕上学位论文 废水处理系统中降解基因的克隆新技术研究及多样性分析 ABSTRACT Heterocyclic aromatic hydrocarbons are major pollutants in industrial wasterwater treatment．Many efficient pollutant treating techniques have been reported，but we know little about the microbial major functional species and degrading—related genes．In this study,molecular microbial ecology technology and genomic technology were used to analyze the microbial communities in heterocyclic aromatic hydrocarbons(such as quinoline)containing industrial wasterwater treatment．Functional species were studied and a promoter trap plasmid system WAS constructed for cloning degrading·related genes． Quinoline is the typical pollutants in industrial wastetwater treatment．Due to its strong water solubility,it has become widespread pollutant．We had set up a lab scale denitrifying Quinoline-degrading bioreactor．After 1 2 months of acclimation，it reached lligh quinoline removal efficience of about 80％．The study showed that in the process of bioreactor,there are hi．gh diverse bcrA and oxoO genes at the bioreactor．The number of bacteria with oxoO gene decreased while the bacteria with bcrA gene increase gradually during the miming of the bioreactor．It indirectly proved that bcrA gene participates in the anaerobic degradion of quinoline．Because the bundance of these functional genes closely related wim the degradation rate in the process of bioreactor,we call use these two genes 勰a biomarker to monitor the quinoline degradation in wastewater treatment system． In order to intensively investigate the functional genes related to heterocyclic aromatic hydrocarbons degrading,a promoter trap plasmid system was constructed．The screening method was initially explored for cloning gene fragments with different promoter activity from the wasterwater treatment system based on the su