餐厨垃圾发酵产l乳酸工艺优化研究环境工程专业论文.docxVIP

万方 万方数据 河北科技大学硕士学位论文 II AbstractLactic Abstract Lactic acid which belongs to the basic chemical raw materials for the production of bom commodity and specialty chemicals is acknowledged as one of the most widely USed organic acids worldwide．At present，95％of the L-lactic acid is obtained from food production．Food waste is recognized as a typical kind of raw material which has the chatacteristics of large quantity，extensive sources and abundant organic matter，and it Can be the raw materials for the production of L-lactic acid．Food waste was taken as the object in this study．On the basis of screening advanced lactic acid bacteria，pretreatment of enzymatic hydrolysis on food waste and cooperative fermentation by corn stalk were c剐Tied out to oDtimize conditions of L-lactic acid production from food waste．The main research results were summarized as follows： 1、An advanced lactic acid bacteria was isolated from plain yogurt on MRS agar· After identified by normally physiological and biochemical tests，the bacteria could be a homofe衄entative Lactobacillus plantarum species．It growed quickly and 6～9h was the logarithmic Dhase．In addition，its lactate production capacity could be up to 1429‘L～· 2、The results of the pretreatment of enzymatic hydrolysis on food waste showed that the oDtimum proportion of enzyme preparation was 450U‘gVS。1 of a’amylase， 1 80U．gVS。1 of lipase and 90U·gVS—of trypsin，respectively．And the optimum hydrolysis condition was 45。C of temperature，5．0 of pH value，and 60min of hydrolysis time．In this condition，the maximum yield of L．1actic acid was 0．2469’gVS叫，which was enhanced by 86．4％more than that of the untreated food waste． 3)The results of fermentation of food waste pretreated by enzymatic hydrolysis sbowed that the optimum parameter was 45。C of temperature，6．0 of initial pH value，l：6 of solid．1iquid ratio，and 1 0％of inoculum size．Under this condition，the maximuIn yield of L．1actic acid was 0．3259·gVS—after 24-hour fermented． 41 The results of cooperat