离子色谱-电导检测法快速测定乳与乳制品中硫氰酸根含量的研究.PDF

6 3 Vol. 6 No. 3 2015 3 Journal of Food Safety and Quality Mar. , 2015 - * 其布勒哈斯 , 李 红, 王 佳, 马文丽, 宋晓东 ((), 011500) 摘 要: 目的 -方法 , AS16 , 35 mmol/L , , 结果 0.02~20 μg/mL, R=0.9990, 0.1 mg/kg, 0.89%~3.31%, 96%~105%结论 , 关键词: -; ; Study on determination of thiocyanate ion in milk and dairy products by ion chromatography-conductivity detection * QI Bu-Le-Ha-Si , LI Hong, WANG Jia, MA Wen-Li, SONG Xiao-Dong (Inner Monggolia Mengniu Dairy(Group)Company Limited , Hohhot 011500, China) ABSTRACT: Objective To establish a rapid method for the determination of thiocyanate ion in milk and dairy products by ion chromatography (IC) method . Methods The samples were extracted by water and methanol, then centrifuged, supernatant fluid were filtered by filter membrane. The samples were analyzed using AS16 column, and elution with the mobile phase of 35 mmol/L potassium hydroxide, and detected by conductivity detector, the external standard was used for quantitative. Results The linearity of the method was 0.02~20 μg/mL (R=0.9990), the limit of detection was 0.1 mg/kg, and the relative standard deviations were 0.89%~3.31%, the average recoveries were 96%~105%. Conclusion This method is a rapid, accurate, and of high precision method, and is suitable for the requirements of the thiocyanate ion analysis. KEY WORDS: ion chromatography-conductivity te