降钙素原荧光免疫层析方法建立.PDF

440 生 物 工 程 学 报 刘传志 等/ 降钙素原荧光免疫层析方法的建立 Chinese Journal of Biotechnology /cjbcn Mar. 25, 2018, 34(3): 440−448 DOI: 10.13345/j.cjb.170305 ©2018 Chin J Biotech, All rights reserved ·生物技术与方法· 刘传志，李伟，刘桂莹，杨羽，宫平，侯玥 长春理工大学 生命科学技术学院，吉林 长春 130021 刘传志，李伟，刘桂莹, 等. 降钙素原荧光免疫层析方法的建立. 生物工程学报, 2018, 34(3): 440–448. Liu CZ, Li W, Liu GY, et al. Detection of procalcitonin based on fluorescence immune chromatography. Chin J Biotech, 2018, 34(3): 440–448. 摘 要: 降钙素原 (Procalcitonin ，PCT) 是降钙素的前体物质，与人体细菌感染情况严重程度相关。通过 PCT 抗体与量子点偶联，自制试纸条，开发一种高灵敏度、快速便捷的 PCT 检测产品，对其初步应用。PCT 单抗效 价达 107 ，PCT 蛋白与量子点偶联，具有较好稳定性。试纸条线性检测范围0.15–120 μg/L ，灵敏度0.007 μg/L ， 回收率范围91%–113% ，批内批间变异系数小于8%。自制的荧光检测试纸条与市场上已有的降钙素原ELISA 试 剂盒进行比对，检测结果良好，基本能够完成临床样品的检测。 : 降钙素原，荧光免疫层析，量子点，试纸条 Detection of procalcitonin based on fluorescence immune chromatography Chuanzhi Liu, Wei Li, Guiying Liu, Yu Yang, Ping Gong, and Yue Hou College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130021, Jilin , China Abstract: Procalcitonin (PCT) is the precursor of calcitonin related to the severity of human bacterial infection. We made a test strip by coupling anti-PCT to quantum dot, in order to develop a highly sensitive and convenient PCT testing product. The anti-PCT titer had reached 107 because of the stability by coupling anti-PCT with quantum dot. The detecting linear range of the experiment was 0.15 to 120 μg/L, the sensitivity was 0.007 μg/L, the recovery range was 91% to 113%, and the intra- and inter-assay coefficient of variation was less than 8%