AOCS脂肪酸检测方法.pdfVIP

SAMPLING AND ANALYSIS OF COMMERCIAL FATS AND OILS AOCS Official Method Ce 1f-96 Reapproved 1997 • Revised 2001 Determination of cis- and trans- Fatty Acids in Hydrogenated and Refined Oils and Fats by Capillary GLC DEFINITION This method consists of the gas–liquid chromatography (GLC) conditions optimized to identify and quantify the trans fatty acid isomers in vegetable oils and fats (References, 1). The fatty acid methyl esters (FAME) of the sample are separated on a capillary gas chromatography column having a high - ly polar stationary phase, according to their chain length (CL), degree of (un)satur ation, and geome - try and position of the double bonds [DB(s)]. SCOPE This method is specially designed to evaluate, by a single capillary GLC procedure, the level of trans isomers as formed during (high-temperature) refining or during hydrogenation of vegetable oils or fats (see Notes, 1 and 2). The method may also be used to report all other fatty acids, for example to obtain saturated fatty acid (SAFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) levels from the same sample and same analysis. APPARATUS 1. Carrier gas—helium, nitr ogen, or hydrogen, GC quali- 1. Gas ch ro m at ograph—equipped with a cap i l l a ry injection ty, dried, and oxygen removed by suitable filters. system (pre fe rably split mode, operated at a split ratio of 2. Internal standard (for calculating fatty acid data as mg ap p rox i m at e ly 1:100) and flame ionization detector per g oil)—t