一株脱色真菌的鉴定及脱色特性的初步探讨-微生物学报.PDF

微生物学通报 JUL 20, 2009, 36(7): 956~961 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 一株脱色真菌的鉴定及脱色特性的初步探讨 梁红昌 千英花 张庆华 陈和淼 吴晓玉* ( 330045) 摘 要: 从废水环境中分离筛选到一株高效染料脱色真菌, 根据形态学及显微特征初步鉴定为泡 盛曲霉(Aspergillus awamori ), 命名为 Asaw117; 从偶氮类、蒽醌类和氧醌类中选取 8 种不同染料 进行脱色分析表明, 该菌株对 0.1 g/L 蒽醌类染料还原蓝 RSN 的脱色率可达 100%; 采用不同培养 基及不同种类碳氮源进行试验比较, 菌株在查氏培养基中生长慢, 但脱色效果最好, 在马铃薯培 养基中生长旺盛, 脱色效果次之。此外, 菌株 Asaw117 能利用还原蓝 RSN 作为氮源, 但不能利用 其为碳源; 几种碳氮源组合实验中, 菌株在蔗糖、硝酸铵组合的査氏培养基脱色效果为好。因此 对处理印染废水具有较好的应用潜力。 关键词: 泡盛曲霉, 鉴定, 脱色, 染料 Preliminary Discuss on Identification and Characterization of One Decolorizing Fungus LIANG Hong-Chang QIAN Ying-Hua ZHANG Qing-Hua * CHEN He-Miao WU Xiao-Yu (College of Bioscience and Bioengineering, Jiangxi Agriculture University, Nanchang, Jiangxi 330045, China) Abstract: According to morphological and microscopic characteristics, a high-efficient decolorizing fungus, the strain Asaw117, was identified as Aspergillus awamori . Selecting eight different dyes from Azo dyes, anthraquinones dyes and oxygen Quinones dyes, the decolorizing assays of various dyes showed that the strain Asaw 117 was the highest decolorizing potential to 0.1 g/L Vat Blue RSN, the discoloration rate up to 100 percent. Comparing to different kinds of medium and several of carbon and nitrogen sources, the strain had the best decolorizing efficient although grew slower in the Czapek medium, otherwise, gre