阔鳞瘤蕨(水龙骨科)配子体形态发育的研究.pdf

25 　1 　　　　　　　　　　　　　　　　　　　　　　 2005 　1 Vol.25　No.1　　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH Jan., 　2005 () 1, 2 1 ＊ 3 2 1 徐艳 　石　雷 　刘保东 　刘　燕 　李　东 (1., 　100093) (2., 　100083) (3., 　150080) 　　利用光学显微镜详细观察记录了阔鳞瘤蕨孢子萌发、配子体发育及幼孢子体形成的整 形态发育过程。结果表明:成熟孢子黄色,极面观椭圆形,赤道面观肾形, 近极单裂缝,表面具大小 不一的球形颗粒。接种后6 ～8 d 孢子萌发, 萌发类型为书带蕨型,形成3 ～7 细胞的丝状体。原 叶体发育为槲蕨型, 接种后30 d 左右进入片状体阶段, 毛状体出现在片状体形成之后, 幼原叶体 短舌状,成熟原叶体心脏形。精子器在接种后60 d 左右开始产生, 颈卵器在精子器发生后10 d 左 右形成,每 原叶体上只能形成一 受精卵。培养基培养的片状体常在基部进行营养繁殖。 　阔鳞瘤蕨;孢子萌发;配子体发育 Observation on the gametophyte development of Phy matosorus hainanensis (Polypodiaceae) 1, 2 1 ＊ 3 2 1 XU Yan 　SHI Lei 　LIU Bao-Dong 　LIU Yan 　LI Dong (1.Ins i u e of Bo any, he Chinese Academy of Science, Beijing 　100093) (2.College of Landscape Archi ec ure, Beijing Fores ry Universi y, Beijing 　100083) (3.Depar men of Biology, Harbin Normal Universi y, Harbin　150080) Abstract 　Spore germina ion, game ophy ic developmen and forma ion of young sporophy es of Phymatosorus hainanensis (Noo .)S.G .Lu have been observed in de ail by he microscope .The resul exhibi s ha he ma ure spore is yellowish,monole e, reniform in equa orial view and ellip ical in polar view, wi h vary sizes global granules on i .The spores germina ed in 6 ～8 days af er inocula ed in he medium, he germina ion is of he Vi aria-ype, and he germ filamen developed 3 ～7cells long .The game ophy e developmen is of he Drynaria-ype, pro halli formed in 30 days af er inocula