台大公共卫生学院-公共卫生学系.PDFVIP

臺大公共衛生學院 職業醫學與工業衛生研究所 104學年度上學期 海報論文研討會論文集 博 士 班 姓名中( /英) 陳其欣 / Chi-Hsin Sally Chen 學號 碩博班 /年級 博二 (如碩二) 論文題目 以質譜技術辨識釩及多環芳香烴碳氫化合物 （中文） 暴露所導致的尿液代謝體變化 論文題目 Identifying urinary metabolic changes with （英文） vanadium and Polycyclic aromatic hydrocarbons exposures using mass-spectrometry-based metabolomics 摘要 Purpose: This study aims to investigate urinary metabolic changes after exposure to petrochemical complex pollutants, vanadium (V) and polycyclic aromatic hydrocarbons(PAHs).Methods: We recruited high exposure groups, who live near a petrochemical complex with higher urinary levels of V and 1-hydroxypyrene (1-OHP; PAHs biomarker),aged 9-15 (n=40) and 55 years (n=70).Low exposure groups lived further away from the complex with lower urinary levels of V and 1-OHP, matched to high exposure groups by age 9-15 (n=71) and 55years (n=71). Urine metabolomes were analyzed following Nature protocol using 2 dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOFMS). 3 online databases were used for characterization: Chemical Entities of Biological Interest (ChEBI), Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Human Metabolome Database(HMDB).Partial least-squares-discriminant analysis (PLS-DA) was used to find metabolic changes between exposure groups. Results: GCxGC-TOFMS extracted 466 peaks. PLS-DA examined metabolic profile variances and found clear separation between exposure groups. On the basis of the variable importanc