改良的在体循环法用于胰岛素的鼻腔吸收研究Modification-药学学报.PDF

药学学报 Acta Pharmaceutica Sinica 20 18, 53 (9): 1551 1556 ·1551 · 良的在体循环法用于胰岛素的鼻腔吸收研究 高明月, 霍英楠, 申 欣, 毛世瑞* (沈阳 科大学 学院, 辽宁 沈阳 110016) 摘要: 本文建立了一种适用于胰岛素鼻腔吸收研究的在体实验方法。首先, 考察在体鼻循环实验方法对胰岛 素的适用性, 结果发现, 胰岛素存在一定的管路吸附, 传统方法不适用于胰岛素的研究; 而在循环液中加入 0.001% Labrasol 可以有效解决胰岛素的管路吸附问题, 该改良方法可用于胰岛素鼻腔吸收研究。采用该方法考 察pH 和药物浓度对胰岛素鼻腔吸收的影响, 结果表明, 相比于pH 4.5 和pH 7.4, 物在pH 6.0 的条件下吸收速 度最低。同时, 胰岛素的鼻腔吸收机制可能是基于浓度梯度的被动扩散。 关键词: 胰岛素; 鼻腔在体循环; 鼻腔吸收; 管路吸附; 表面活性剂 中图分类号: R943 文献标识码: A 文章编号: 0513-4870 (20 18) 09- 1551-06 Modification of in situ perfusion in study of nasal absorption of insulin * GAO Ming-yue, HUO Ying-nan, SHEN Xin, MAO Shi-rui (School of Pharmacy, Shenyang Pharmaceutical University, Shenya ng 110016, China ) Abstract : The obj ective of this study is to develop an in vitro screening method for nasal absorption of insulin. First, the adaptability of in situ rat nasal perfusion test for the study of insulin was investigated. It was found that insulin was liable to be absorbed on the silicone tube and the traditional method is not suitable . However, addition of 0.00 1% Labrasol into the perfusate can effectively solve this problem. A modified method suitable for in situ rat nasal perfusion of insulin was established with the addition of 0.001% Labrasol into the perfusate. Using the modified method, effect of pH and drug concentration on the absorption of insulin in the nasal cavity was further investigated. The re