人工老化对青川产北柴胡种子生理生化特性的影响-天津中草药杂志社.PDFVIP

·844 · 中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 6 期 2014 年 3 月 人工老化对青川产北柴胡种子生理生化特性的影响 1 1* 1 1 1 2 姚入宇 ，陈兴福 ，谌琴琴 ，屈晓霞 ，王 菲 ，杨兴旺 1. 四川农业大学农学院 农业部西南作物生理生态与耕作重点实验室，四川 温江 611130 2. 四川德培源中药科技开发有限公司，四川 绵阳 621000 摘 要：目的 探究青川产北柴胡种子的劣变机制，揭示其不耐贮藏的原因。方法 采用人工老化的方法处理柴胡种子，并 分析老化过程中种子的抗氧化酶活性、电导率和营养物质量变化。结果 随老化时间延长，老化种子的过氧化物酶（POD ）、 超氧化物歧化酶（SOD）、抗氧化酶（CAT ）活性持续下降；可溶性糖和可溶性蛋白的量降低，老化3 d 后均处于低量水平； 种子浸出液电导率下降；经老化处理 1 d 的种子即已丧失萌发能力。结论 青川产北柴胡种子劣变机制包含氧化损伤、膜损 伤和营养物质过度消耗 3 条途径，种子不宜长期贮藏，且贮藏要避免高温、高湿环境。 关键词：北柴胡；种子；人工老化；劣变；贮藏 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2014)06 - 0844 - 05 DOI: 10.7501/j.issn.0253-2670.2014.06.020 Effects of artificial aging on physiological and biochemical characteristics of Bupleurum chinense seeds from Qingchuan county 1 1 1 1 1 2 YAO Ru-yu , CHEN Xing-fu , SHEN Qin-qin , QU Xiao-xia , WANG Fei , YANG Xing-wang 1. Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China 2. Sichuan Depeiyuan Traditional Chinese Medicine Technology Development Co., Ltd., Mianyang 621000, China Abstract: Objective To investigate the deterioration mechanism of Bupleurum chinense seeds from Qingchuan county, thus to study the seeds harvesting and storage characteristics, and to reveal the deterioration mechanism. Methods Artificial aging method is used to study the seeds deterioration and to observe the changes of nutrient substance, anti-oxidase activities, and conductivity of seeds. Results While the seeds deteriorated, their activities of anti-oxidase (POD, SOD, and CAT) decreased, and the content of soluble sugar and soluble protein reduced also as the aging time prolonged. After 3 d treatm