电针“神庭”、“百会”对脑缺血再灌注损伤大鼠认知功能的影响与机制分析.pdfVIP

电针“神庭”、“百会”对脑缺血再灌注损伤大鼠认知功能的影响及机制研究 4 Western blot 及RT-PCR 观察大鼠额叶皮质脑缺血组织NF-κB-p65 、Bax 及Fas 转录及翻译水平 模型组大鼠脑缺血组织中NF-κB-p65 、Bax及Fas蛋白印迹和 mRNA扩增产物电泳 条带光密度较假手术组明显升高，差异具有统计学意义（n=6 ，P0.05 ）。与模型组相 比，电针组大鼠额叶皮质组织中NF-κB-p65 、Bax及Fas蛋白印迹和 mRNA扩增产物电 泳条带光密度明显降低，差异具有统计学意义（n=6 ，P0.05 ），说明通过抑制NF-κB 信号通路介导的细胞凋亡可能是电针改善脑缺血再灌注损伤大鼠空间学习、记忆能力 及其具有神经保护作用的分子机制之一。 结论 1 电针“神庭”、“百会”可以改善局灶性脑缺血再灌注损伤大鼠的认知功能； 2 电针“神庭”、“百会”对局灶性脑缺血再灌注损伤大鼠具有神经保护作用； 3 通过调控额叶皮质组织NF-κB-p65 、Bax 及Fas 转录及翻译水平从而抑制细胞凋 亡可能是电针改善模型大鼠认知功能的分子生物学机制之一。 关键词 电针；脑缺血再灌注；认知功能；细胞凋亡 2 摘要 The effeacy and the mechanisms of electroacupuncture at “Shenting” and “Baihui” on the cognitive impairment in cerebral ischemia-reperfusion injured rats LiuJiao (Rehabilitation Medicine and Physiatrics) Directed by Feng Xiaodong Abstract Objective Through a focal cerebral ischemia-reperfusion (I/R) injury rat model, we evaluated the therapeutic efficacy from the ethology and the mechanisms from the histopathology/molecular boilogy and other kinds of modern experiment technology of electroacupuncture at “Shenting” and “Baihui” against the cognitive impairment. Methods There were 60 male SD rats which were diveded into the sham operation control group (SC), ischemia control group (IC) and electroacupuncture group (EA) randomly, 20 rats in each group. Using the focal cerebral ischemia-reperfusion (I/R) rat model, in the present study we made the cognitive impairment model after stroke, then the rats were administered electroacupuncture at “Shenting” and “Baihui” for 30min daily for 10 days in the EA group. we evaluated the