dynamic light scattering measuring platelet qual发ity and bacterial 动态光散射测量血小板质量和细菌.pptVIP

Dynamic Light Scattering Measuring Platelet Quality and Bacterial ContaminationElisabeth Maurer, Ph.D. Canadian Blood Services, RD VancouverUBC, Department of Pathology and Laboratory MedicineUBC Centre for Blood Research Thank You! Keddie Brown, iCapture Audrey Labrie, CBS Dr. Mark Scott, CBS Dr. Kate Chipperfield, VGH Cheryl Pittendreigh, CBS Nobu Kitamura, CBS CBS Intellectual Property, Intramural and Bridge Funding * * The Problem Platelet transfusions used to treat and/or prevent bleeding Number of platelet transfusions produced per year: Canada: 300,000 United States: 2,000,000 Europe: 2,500,000 Platelets are sensitive to stress (e.g., cold damage) Activated platelets do not help patients. Storage at 22oC to prevent cold damage Increased risk for bacterial growth Shortages - addressed by increasing donor recruitment Outline The Problem Clinical Situation Solving the problem Novel Approach Data Acquisition DLS-PM score The Invention Technology Background The DLS-PM Accomplishments Clinical Results Summary Other Technology Accuracy, Sensitivity No non-invasive in vitro test available Tested inventory cannot be transfused Platelet quality / bacterial testing cannot be mandated Good outcome ? Assumption: good platelets Bad outcome ? Assumption: bad platelets Transfusion outcome serves as retrospective platelet quality indicator. The Problem: Clinical Situation The Problem: Summary at least 20% discard rate Additional 10% discarded after testing (invasive technology) ? Unknown quality of transfused platelets $1,000 / transfusion ? Worldwide loss of at least 1 billion dollars per year In summary: Unknown platelet quality is a safety issue Discarded platelet transfusions are a financial burden Multi-dimensional quality measurement DLS-PM detects discoid platelets activated platelets microparticles bacteria at the same time Solving the Problem: Novel A