燕麦葡聚糖合酶基因AsCSLH的克隆及特征分析-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2011, 37(4): 723−728 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.00723 燕麦葡聚糖合酶基因AsCSLH 的克隆及特征分析 吴 斌 张宗文* , 100081 : β-, β- β-, RACE β- AsCSLH , RT-PCR CSLH 6 , GT/AG , 2 277 bp, CSLH , AsCSLH , AsCSLH β- : ; β-; ; Cloning and Analysis of β-Glucan Synthase Gene AsCSLH in Avena sativa L. * WU Bin and ZHANG Zong-Wen Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China Abstract: Beta-glucan [(1→3,1→4)-β-D-glucan] is one of the important components in oat (Avena sativa L.) that has the function of health protection for human beings. Cloning the β-glucan synthase gene is of significance to reveal the molecular mechanism of β-glucan accumulation and guide oat breeding for β-glucan enhancement. A β-glucan synthase gene, designated AsCSLH , and its genomic DNA sequence were cloned from the oat landrace Xiayoumai using rapid amplification of cDNA ends (RACE) and Ge- nomeWalk from the cDNA, respectively. The AsCSLH gene contains a 2 277 bp open reading frame and encodes a peptide of 758 amino acid residues. Six introns were found in AsCSLH gene with typical GT/AG characteristic after comparison of its sequences of genomic DNA and cDNA. Sequences analysis showed that AsCSLH gene shared the highest identity with rice CSLH gene (BK000084). Structure analysis of predicted encoded protein also confirmed that this cDNA sequence was the oat β-glucan synthase gene. The tissue-specific expression analysis by semiquantitative RT-PCR assays suggested that AsCSLH was expressed in different tissues of oat with the hig