鸭茅种质遗传变异及亲缘关系的SSR分析-中国科技论文在线.PDFVIP

中国科技论文在线 遗 HEREDITAS(Beijing)2009年6月，31(6)：654--662 ISSN 0253—9772 WWW．chinagene．ca 研究报告 DOI：10．3724／SP．J．1005．2009．00654 鸭茅种质遗传变异及亲缘关系的SSR分析 谢文刚，张新全，马啸，彭燕，黄琳凯 四川农业大学草业科学系，雅安 625014 摘要：利用SSR标记技术对来自世界5大洲的53份鸭茅(Dactylis glomerata L．)材料的遗传变异及亲缘关系进 行了研究 用筛选的l5对引物对53份材料的DNA进行PCR扩增，获得下述结果：(1)15个位点共检测到127 个等位基因，每个位点等位基因变幅为5～l2个，平均为8．5个，多态性位点率(P)平均为95．21％；多态性信息 含量(PJc)范围为0．30(A04C24)到 0．44(A0IF24)，平均为0．36；(2)材料问遗传相似系数(G )范围在0．43到0．94 之间；地理类群间的GS值在0．73到0．91间，其中亚洲(P，90．55％)和欧洲(P，86．61％)类群遗传多样性丰富，表明 供试鸭茅种质具有丰富的遗传变异；(3)根据研究结果进行聚类分析和主成分分析，可将53份鸭茅材料分成5大 类，来自相同洲的材料能聚为一类，呈现出一定的地域性分布规律。 关键词： 鸭茅；种质资源；SSR；遗传多样性 Genetic variation and relationship‘enetic anel relationsII in orcIIar~rass Oactvlts ’ dd (Dactylis glomerata L．)germplasm detected by SSR markers XIE Wen—Gang，ZHANG Xin—Quan，MA Xiao，PENG Yan，HUANG Lin—Kai Department ofGrassland，Sichuan Agricultural University，Ya’an 625014，China Abstract：Genetic variation and relationship of 53 accessions of D．glomerata collected from 5 continents were analyzed using simple sequence repeat(SSR)molecular markers with 15 SSR primer pairs．The following results were obtained．(1) A total of 127 alleles were detected at 15 loci．The number of alleles per locus ranged from 5 to 12，with an average of 8．5． The rate of polymorphic sites(P)was 95．21％；the polymorphic information content(PIC)ranged from 0．30(A04C24)to 0．44(A01F24)with an average of 0．36．(2)The genetic similarity(GS)among all accessions ranged from 0．43 to 0．94，for all geographical groups GS ranged from 0．73 tO 0．9 1，and high genetic diversity was observed in Asia(P，90．55％)and Europe (尸，86．61％)groups．These results suggested that there was rich genetic diversity among all orchardgrass accessions tested． (3)Based on the cluster and principal component analyses，53 accessions could be divided into five groups according to the nearest ph