不可逆电穿孔对兔组织阻抗的影响-高电压技术.PDF

第 41 卷 第 4 期：1402-1408 高电压技术 Vol.41, No.4: 1402-1408 2015 年 4 月 30 日 High Voltage Engineering April 30, 2015 DOI: 10.13336/j.1003-6520.hve.2015.04.045 不可逆电穿孔对兔组织阻抗的影响 董守龙，姚陈果，储贻道，米 彦，李成祥，赵亚军 400030 （重庆大学输配电装备及系统安全与新技术国家重点实验室，重庆 ） 摘 要：为了研究不可逆电穿孔对生物组织阻抗的影响，探究了阻抗变化与不可逆电穿孔机理之间的关系。以新 西兰大白兔的多种组织作为对象，对其施加 μs 级高强度脉冲电场，测量、计算了组织阻抗值的变化。实验采用脉 冲参数为：幅值 2 250 V ，脉冲脉宽 70 μs，重复频率2 Hz ，脉冲总数 90 个。实验结果表明：生物各组织发生不 可逆电穿孔时，其阻抗均减小；随着脉冲作用时间增加，阻抗趋于稳定，但在 41 个、61 个脉冲作用后分别停止 5 min 后阻抗明显恢复，说明可逆电穿孔比例仍较大；随着脉冲电场强度和作用时间的继续增大，不可逆电穿孔数 量和程度迅速增大，直至组织阻抗达到最小值；停止施加脉冲电场后，组织阻抗增大，即部分电穿孔自恢复。 关键词：不可逆电穿孔；阻抗；电脉冲；电导率；细胞膜；组织消融 Influence of Irreversible Electroporation on Tissues Resistance of Rabbit DONG Shoulong, YAO Chenguo, CHU Yidao, MI Yan, LI Chengxiang, ZHAO Yajun (State Key Laboratory of Power Transmission Equipment System Security and New Technology, Chongqing University, Chongqing 400030, China) Abstract ：In order to study how irreversible electroporation influences vivo tissue impedance, we experimentally studied the relationship between impedance change and irreversible electroporation mechanism. Taking the several tissues in New Zealand white rabbits as the research object, we treated them with nanosecond pulsed electric field, and measured the change of vivo tissue impedance. The applied pulsed electric field was with amplitude of 2 250 V, pulse width of 70 μs, and repetitive frequency of 2 Hz, and the total pulse number was 90. Conclusions are drawn as follows. Due to irreversi- ble electroporation, the resistances of all the tested vivo tissues wi