亚临界水状态下制备共轭亚油酸甘油酯.PDF

12 2013, Vol.34, No.08 食品科学 ※工艺技术 亚临界水状态下制备共轭亚油酸甘油酯 王 雪，李万振，宋 鹏，刘 鑫，于殿宇* (东北农业大学食品学院，黑龙江 哈尔滨 150030) 摘  要：在亚临界水的状态下，以共轭亚油酸(CLA)和甘油为原料，采用直接酯化法合成CLA甘油酯。通过单因素试 验，考察反应温度、反应时间、反应物物质的量比(CLA:甘油)、反应压力对CLA转化率的影响；并在单因素水平的基 础上，进行响应面优化，确定反应的最佳条件为反应温度250 ℃、反应时间40min 、反应物物质的量比2:1、反应压力 13MPa，在优化出的最佳条件下进行反应，得到的CLA转化率为96.3%，所得CLA甘油酯为淡黄的油状透明液体。 关键词：亚临界水；共轭亚油酸(CLA) ；共轭亚油酸甘油酯(CLA甘油酯) ；CLA转化率 Preparation of Conjugated Linoleic Acid Glyceride in the Presence of Subcritical Water * WANG Xue ，LI Wan-zhen ，SONG Peng，LIU Xin ，YU Dian-yu (College of Food Science, Northeast Agricultural University, Harbin 150030, China) Abstract ：Direct esterification method was used to prepare conjugated linoleic acid glycerides from conjugated linoleic acid (CLA) and glycerin under the condition of subcritical water. Effects of temperature, time, reactant molar ratio (CLA:glycerin), pressure on conversion rate of CLA was studied. Through single factor test and response surface optimization, the optimum reaction conditions were obtained: Reaction temperature was 250 ℃, reaction time was 40 min, substrate molar ratio was 2:1, reaction pressure was 13 MPa. The conversion rate of CLA observed under the optimum conditions was 96.3%, and the resulting CLA glyceride was a yellow, oily, transparent liquid. Key words ：subcritical water ；conjugated linoleic acid (CLA) ；conjugated linoleic acid glyceride (CLA glyceride) ； conversion rate of CLA 中图分类号：TS229 文献标志码：A 文章编号：1002-6630(2013)08-0012-04 共轭亚油酸(conjugated linoleic acids，CLA)是亚油酸 中溶解度低的疏水性有机物具有良好的溶解能力[13] ，在 的一组位置和几何异构体的通称。CLA属具有较高生理 此状态下，CLA和甘油可溶解其中，进行充分反