用β-紫罗兰酮筛选虾青素高产菌株-微生物学报.PDF

微生物学通报 NOV 20, 2008, 35(11): 1828~1832 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 生物实验室 用β-紫罗兰酮筛选虾青素高产菌株 1 1,2 1,2 1,2 1,2* 伊文刚 肖安风 李利君 倪 辉 蔡慧农 (1. 361021) (2. 361021) 摘 要: 用 β- 紫罗兰酮作为筛选剂选择性分离海洋红酵母虾青素高产突变菌株。实验结果表明, 在 β-紫罗兰酮存在的情况下, 由于类胡萝卜素合成受到抑制, 海洋红酵母的生物量和虾青素合成 量都减少; 当平板培养基中β-紫罗兰酮浓度达到370 mg/L 时, 海洋红酵母的致死率为92.3%; 在 这种平板培养基上涂布经甲基磺酸乙酯诱变的海洋红酵母, 随机筛选 200 个菌落, 结果表明生物 量、虾青素体积产率和细胞产率均有所提高的正突变株占 18%, 生物量、虾青素体积产率和细胞 产率的单项指标有所提高的正突变株分别占 22.5% 、45%和 46% 。该实验结果表明在分离培养基 中添加 β- 紫罗兰酮可选择性地分离海洋红酵母虾青素高产菌株, 提高虾青素高产突变株的筛选 效率。 关键词: 海洋红酵母, 虾青素, β-紫罗兰酮, 高产菌株, 筛选模型 Screening Astaxanthin-hyperproducing Strains with β-ionone YI Wen-Gang1 XIAO An-Feng1,2 LI Li-Jun1,2 NI Hui1,2 CAI Hui-Nong1,2* (1. School of Biotechnology Engineering, Jimei University , Xiamen 361021) (2. Research Center of Food Biological Engineering Technology of Xiamen City, Xiamen 361021) Abstract: The astaxanthin-hyperproducing mutants of oceanic red yeast were efficiently screened with β-ionone. As shown by the result, the biomass and astaxanthin content of oceanic red yeast was reduced due to the presence of β-ionone which inhibited the synthesis of carotenoids. The lethality rate of oceanic red yeast would attained 92.3% if 370 mg/L of the β-ionone concentration was contained in the plate medium. The oceanic red yeast mutated by ethyl methane sulfonate was cultured on plate m