双相萃取-液相色谱检测细胞培养液样品中的山银花黄酮类化合物.pdfVIP

４７ ２ Vol．４７ No．２ ２０１８ ２ Applied Chemical Industry Feb．２０１８ - ，，，，，， （ ， ４２１００１） ：-， ， C ，-（５０％∶５０％ v／v， pH ４．０ ）， １８ １．０ mL／min，３５２ nm。 ，，５ ， ； ，-， ，、、 、。 ：；／；； ：TQ ０２８ ：A ：１６７１ －３２０６（２０１８）０２ －０４１０ －０３ Flos Lonicerae GUO Yu，ZHU Peng ，LIU Gang ，YAO Xu，LING Shan-cun，DING Lan，LI Chong （Institute of Pharmacy and Pharmacology，University of South China，Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study，Hengyang ４２１００１，China） ：The Flos Lonicerae flavonoids in cell culture mediumin was extracted by the ethanol-K HPO ２ ４ two-phase conditions and its content was detected by HPLC with a C column．Samples were eluted using １８ methanol／water （５０％∶５０％ v／v） with phosphoric acid （apparent pH４．０） at a flow rate of １．０ mL／min and detected at ３５２ nm．Results showed that，with the two-phase system extracting flavonoids crude extract from Flos Lonicerae，chromatographic behavior of the ５ obvious signals from the upper phase sample were almost the same as the flavonoids crude extract samples．When the samples of flavonoids crude extract from Flos Loniceraein cell culture medium were extracted by the sameprocedure，test results were also ba- sically the identical．All these suggest the ethanol-K HPO two-phase system combined with HPLC can be ２ ４ determined the Flos Lonicerae flavonoids in cell culture medium samples and the two-phase system can be better extraction and concentration of