实时定量荧光PCR快速鉴定食品中单核细胞增生李斯特氏菌.PDF

8 1 Vol. 8 No. 1 2017 1 Journal of Food Safety and Quality Jan. , 2017 PCR 1 2 2 2 1 1 1* 刘万静 , 刘 斌 , 李湘平 , 李海燕 , 石 红 , 吴友伟 , 答 嵘 (1. , 710061; 2. , 725000) 摘 要: 目的 PCR (real-time PCR)(Listeria monocytogenes, LM)方法 2016 134 LM 10 , GB/T4789.30-2010 real-time PCR 结果 134 , 8 LM, 5.97%GB/T4789.30-2010 , real-time PCR LM 100%LM real-time PCR 100%, 0%结论 , , , 关键词: ; PCR; Rapid detection of Listeria monocytogenes in food by real-time PCR 1 2 2 2 1 1 1* LIU Wan-Jing , LIU Bin , LI Xiang-Ping , LI Hai-Yan , SHI Hong , WU You-Wei , DA Rong (1. Department of Clinical Laboratory, The First Affiliated Hospital of Xi’anJiaotong University , Xi an 710061, China; 2. Department of Microbiology Laboratory, Center for Disease Control and Prevention of Ankang, Ankang 725000, China) ABSTRACT: Objective To establish the method for rapid identification of Listeria monocytogenes (LM) in food by real-time PCR. Methods One hundred and thirty-four cases of national food risk monitoring samples in 2016 and 10 cases of simulated inactivated LM samples were simultaneously detected by GB/T4789.30-2010 method and real-time PCR method. Results A total of