联合提取铁皮石斛中生物碱及多糖的研究.docxVIP

联合提取铁皮石斛中生物碱及多糖的研究 第 PAGE \* Arabic \* MERGEFORMAT 12 页 联合提取铁皮石斛中生物碱及多糖的研究 原　琳1，李　娇1，荣永海1，仝战旗2，荣　龙1收稿日期： 收稿日期：xxxx-xx-xx 接受日期：xxxx-xx-xx *通讯作者 Tel: 86-010 E-mail: ronglong@buaa.edu.cn 1北京航空航天大学生物与医学工程学院,北京 100191；2解放军总医院，北京 100853 摘要为提高铁皮石斛有效成分的综合利用效率,本研究利用闪式提取法对其中的生物碱、结合多糖与游离多糖进行了联合提取，并对酶解条件进行了优化。生物碱、结合多糖及游离多糖的得率分别为0.136%、4.2%及19.3%；纯度分别为78.2%、81.3%及90.6%。通过单因素试验所确定的最佳酶解条件为：料液比1:30、纤维素酶添加量32IU/g、木瓜蛋白酶添加量39200U/g、酶解时间2.5h。利用GPS测定所提取的结合多糖及游离多糖的重均分子量分别为2373Da、17987Da。本研究确定了利用铁皮石斛联合制备生物碱及多糖的工艺，本工艺方法简便、成本低廉，适于工业化生产。 关键词铁皮石斛；联合提取；生物碱；多糖 中图分类号：R284.2 文献标志码：A Study on Combined Extraction of Alkaloids and Polysaccharides from Dendrobium officinale YUAN Lin1，LI Jiao1，RONG Yong-hai1，TONG Zhan-qi2, RONG Long1* 1School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China； 2The general hospital of Chinese Peoples LiberationArmy, Beijing, 100853, China Abstract: To improve the comprehensive utilization rate of Dendrobium officinale, this study explored a method to extract alkaloids and polysaccharides jointly from Dendrobium officinale. Taking Dendrobium officinale as materials, alkaloid, joined and free polysaccharide were combined extracted using flash extraction technology. And the enzymatic hydrolysis conditions were optimized. The yields of alkaloid, joined and free polysaccharide were: 0.136%, 5.2% and 24.3%, respectively. And the purity were: 78.2%, 81.3% and 90.6%, respectively. The optimum hydrolysis conditions determined by single-factor test were: solid-liquid ratio 1:30, the amount of cellulase added 32IU/g, the amount of papain added 39200U/g, the hydrolysis time 2.5h. Weight-average molecular weights of joint and free polysaccharides were 2373Da and 17987Da, determined by GPS.This study developed a combined extraction process of alkaloids and polysaccharides from Dendrobium officinale. The process is simple, low cost, and suitable for promotion. Key words: Dendrobium officinale