基于硒化镉量子点磁微球微悬臂梁式免疫传感器片上磁分离.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2008, February 25; 24(2): 315-322 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究简报 , 1 1 2 1 2 高 鹏 , 姚素英 , 张兵波 , 李二茂 , 常 津 1 , 300072 2 , 300072 : 应用了以硒化镉量子点为荧光探针, 具有磁性和抗体双重靶向功能的聚苯乙烯磁微球。设计了基于此种磁微 球的新型微悬臂梁式免疫传感器, 满足在液相环境中, 借助嵌入到聚苯乙烯磁微球的荧光探针及微球表面的特异性抗 体探针, 达到生物分子的定性检测, 借助具有纳米机械响应的微悬臂梁及微平面电感线圈, 达到生物分子的定量检测 及传感器的复用性, 解决传统微悬臂梁式免疫传感器的不足。着重对三种粒径尺寸的硒化镉量子点进行了表征, 同时 针对片上磁分离的机理, 梁上微电感线圈的结构, 微磁场对磁微球的吸引进行了研究, 设计并优化出满足新型微悬臂 梁式免疫传感器所需的蛇形微平面电感线圈。通过生物磁分离实验, 验证了设计及优化的结果, 实现了用于生物分子分 离的片上磁分离技术。 : 免疫传感器, 硒化镉量子点标记的磁微球, 片上磁分离 On-chip Magnetic Separation of Microcantilever Immuno- sensor Based on the CdSe QDs-tagged Magnetic Microbead 1 1 2 1 2 Peng Gao , Suying Yao , Bingbo Zhang , Ermao Li , and Jin Chang 1 School of Electronic and Information Engineering, Tianjin University, Tianjin 300072, China 2 Institute of Nanobiotechnology , Tianjin University, Tianjin 300072, China Abstract: We designed a novel microcantilever immuosensor based on magnetic microbead, applying different-sized CdSe QDs as fluorescent probes and polystyrene magnetic microbead. The novel microcantilever immuosensor used fluorescent probes embedded polystyrene microbeads and specific antibodies on the surface of the polystyrene microbead. In addition, we studied the mechanism of the on-chip magnetic separation, the structure of micro-electromagn