寒潮诱发高血压大鼠脑卒中与脑血管NF-κB和ICAM-1的表达关系-神经内科学专业论文.docxVIP

PAGE PAGE 10 不同血压水平大鼠脑动脉的NF-κB和ICAM－1表达不同， P0.01；非寒潮组与 寒潮组的表达不同， P0.01；血压与处理方法有交互作用。进一步分析表明：1） 剔除脑卒中的各血压组大鼠经人工气候箱处理后NF-κB和ICAM－1蛋白表达变 化趋势一致如下：在220mmHg血压组，寒潮组的蛋白表达比非寒潮组高， P0.05；在≥220mmHg血压亚组，寒潮组的上述指标比非寒潮组有降低，P0.01； 在非寒潮组，蛋白表达均随血压升高而升高，P0.01。在寒潮组，上述指标在220 mmHg时均随血压升高而升高，P0.05。2）脑卒中后的血浆NF-κB和ICAM－1 蛋白表达均比非卒中组高：a) 脑梗死组比非脑卒中组和脑出血组高，差异有统 计学意义，P﹤0.05，P﹤0.01；b)脑出血组与非脑卒中组脑血管的NF-κB和ICAM －1比较差异均有统计学意义，P﹤ 0.05。 结论 NF-κB和ICAM-1的表达变化可能在高血压脑卒中和寒潮诱发脑卒中的过 程中起着重要作用。 关键词：人工寒潮；高血压；脑卒中；细胞间粘附分子；核转录因子；大鼠 The activity changes of NF-κB and ICAM-1 of Stroke on Renovascular Hypertensive Rats Induced by Artificial Cold Exposure ABSTRACT Objective We have employed the rats with levels of different blood pressure to study the activity changes of NF-κB and ICAM-1 in endothelial dysfunction under ACE. In addition, 2k2c RHR with different levels of high blood pressure were examined to evaluate the contribution of endothelial cell adhesion molecules on the induction of stroke under the cold exposure. Methods Two-kidney-two-clip RHR were used in the present study. The sham-operated rats were undergone the same experimental procedures except for placement of clip, they (n= 24) served as normotensive controls. Normal sham-operated rats and 2k2c RHR were randomly assigned into 2 groups: ACE and non-ACE. Each group was further divided into 4 sub-groups according to their SBP (after 2k2c operation before the ACE treatment): ① Sham-operated control group, BP140mmHg, ②mild hypertensive group, BP of 160-200mmHg, ③moderate hypertensive group, BP of 200-220mmHg, and ④severe hypertensive group, BP≥220mmHg. To establish the ACE and non-ACE treatment, the RHR were housed individually in a modified intelligent artificial climate cabinet. The ACE was designed as 3 cycles of 12 h light of 22℃ and 12 h dark of 4℃. The non-ACE group was kept at 22 ℃ throughout the experiment. Movement of limbs and consciousness of the rats were recorded twice daily during ACE and non-ACE treatment. Systolic BP (SBP), body weight w