海燕内脏脑苷脂的分离纯化及抗肿瘤活性研究-食品科学专业论文.docxVIP

PAGE PAGE IV Abstract lactate dehydrogenase(LDH) in human breast cancer cells (MCF-7) culture fluid were basically consistent with the results of MTT assay, and the larger dose of cerebrosides were, the more LDH in cells leaked and the higher the activity of LDH was. It indicated that cerebrosides increased the membrane permeability of MCF -7 cells effectively. 5. Anti-tumor mechanism of cerebrosides was studied from the perspective of apootosis. Through the morphological observation(inverted microscope and fluorescent staining), cerebrosides decreased the number of MCF-7 cells, rounded cell shape and decreased cell diopter, accompaning apoptotic features about chromatin shrinked, nuclei showed a dense stain and apoptotic bodies. Flow cytometry analysis showed that cerebrosides could induce MCF-7 cells apoptosis and their cell cycle was arrested in the G2/M phase. According to the results of mitochondrial transmembrane potential, cerebrosides deceased mitochondrial transmembrane potential of MCF-7 cells, indicating that cerebrosides inducing MCF-7 cells apoptosis was achieved by regulating the permeability of mitochondria. Key Words: Asterina pectinifera offal; cerebrosides; separation and purification; structural analysis; anti-tumor activity 目录 目录 目录 摘 要 I HYPERLINK \l _bookmark0 第一章 绪论 1 HYPERLINK \l _bookmark1 1.1 海星及脑苷脂简介 1 HYPERLINK \l _bookmark2 1.1.1 海星简介 1 HYPERLINK \l _bookmark3 1.1.2 脑苷脂简介 1 HYPERLINK \l _bookmark4 1.2 脑苷脂的国内外研究现状 2 HYPERLINK \l _bookmark5 1.3 脑苷脂的提取分离 2 HYPERLINK \l _bookmark6 1.4 脑苷脂的含量测定 3 HYPERLINK \l _bookmark7 1.5 脑苷脂的结构及其鉴定方法 4 HYPERLINK \l _bookmark8 1.6 脑苷脂的生物活性 5 HYPERLINK \l _bookmark9 1.7 本课题的研究内容及意义 7 HYPERLINK \l _bookmark10 1.7.1 研究内容 7 HYPERLINK \l _bookmark11 1.7.2 研究意义 7 HYPERLINK \l _bookmark12 1.7.3 技术路线 8 HYPERLINK \l _bookmark13 第二章 海燕内脏脑苷脂的提取、分离及纯化 9 HYPERLINK \l _bookmark14 2.1 材料与仪器 9 HYPERLINK \l _bookmark15 2.1.1 实验材料 9 HYPERLINK \l _bookmark16 2.1.2 实验仪器 9 HYPERL