无刺红树莓组织培养快速繁殖技术研究-吉林农业大学学报.PDF

DOI :10.13327/j.j jlau.2006.04.015 　28 4 　　　　　　　 Vol.28 No.4 　 　2006 8 Journal　of 　Jilin　Agricultural　University August 2006　 朴日子, 曹后男, 陈艳秋, 宗成文, 陈　蕾 (延边大学农学院, 龙井133400) 　:以无刺红树莓带芽茎段和茎尖为外植体, 筛选 组织培养阶段高频、稳定的适宜培养基。 结果表明: 外植体以茎段为佳。 分化培养基以1/2 MS+6-BA 1.0 mg/L+活性炭0.1%最好, 分化率可达100%;继代增殖 培养基以MS+6-BA 1.0 mg/L+NAA 0.1 mg/L 最理想, 增殖倍数可达7 倍;生根培养基以 1/2 MS+IBA 1.0 mg/L+活性炭 1.0 g/L 最佳, 生根率可达93.33%。 生根类型多为皮部生根。 采用2 步移栽法进行移裁 时, 第1 次移裁以河砂为基质移栽成活率达到98%, 第2 次移裁成活率100%。 :无刺红树莓;茎段;组织培养;激素 :S663.2　　　:A　　　:1000-5684(2006)04-0411-04 Study on Tissue Culture and Rapid Propagation of Thornless Red Raspberry Idaeus PIAO Ri-zi, CAO Hou-nan, CHEN Yan-qiu, ZONG Cheng-wen, CHEN Lei (College of Agriculture, Yanbian University , Longj ing 133400, China ) Abstract:Using stem segmentswith buds and ti s as ex lants, tube ra id ro agation of Red Ras berry was studied.The results indicated that the a ro riate media for res ective culture stages were:for ger- mination of auxiliary buds:1/2 MS+6-BA 1.0 mg/L;for differentiation and multi lication:MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, the multi le for multi lication was u to 7;for rooting:1/2MS+IBA 1.0 mg/L+active carbon 1.0 g/L and the rate of rooting was 93.33%.After 20-day acclimatization, the rooted lantletswere trans lanted to substrata in two successive ste s, the survival rates of whichwere 98%and 100 %res ectively. Key words:thornless red ras berry;stem;tissue culture;hormone 　　(Rubus idaeus ), , 。、、 , , , , , , , 。 [3] 3 。,