大麦黄矮病毒蚜传蛋白中蚜传功能位点分析-河南农业科学.PDFVIP

河南农业科学, 2011, 40( 8) : 134- 137 Journal of Henan Agricultural Sciences 吴兴泉, 陈士华, 张苗青, 刘应举 ( , 4 0001) : 为探讨大麦黄矮病毒( BYDV) 蚜传( AT) 蛋白参与介导蚜虫传播BYDV 过程的关键功能区, 利用Clustal 等多 序列分析工具对BYDV AT 蛋白进行综合分析结果显示, 在 GAV 株系 MAV 株系和PAV 株系的AT 蛋白通读蛋白区均具有高度保守序列V DSS和 KRFFEY, 两保 守区之间的氨基酸序列在株系间存在特异性变异, AT 蛋白的主要糖基化和磷酸化位点亦分布在 该区, 表明该区域可能是决定株系间蚜传特性差异的功能区另外分析表明, AT 蛋白具有卷曲螺 旋结构, 在动物细胞中主要定位于细胞核内, 其第 10 位氨基酸残基附近的卷曲螺旋结构不但在序 列上高度保守( EYEAA) 而且位置上也高度相近, 说明该结构可能在 AT 蛋白介导蚜虫传播过程 中起调控基因表达或分子识别的作用 : 大麦黄矮病毒; 蚜传蛋白; 株系; 分子识别; 功能位点 + : S43 . 121. 4 9 : A : 1004-3268( 2011) 08-0134-04 A nalysis of Functional Sites in Aphid Transmission Protein of Barley Yellow Dw arf Virus WU Xing-quan, CHEN Sh-i hua, ZHANG M iao-qing, LIU Ying-ju ( College of Biotechnology , H enan University of Technology, Zhengzhou 4 0001, China) Abstract: In order to know the key functional regions in the aphid transmission ( AT ) protein w ith w hich aphids transmit barley yellow dw arf virus ( BYDV) , BYDV AT protein w as analyzed w ith many sequence analysis tools. The results show ed that there w ere two highly conservative sequences VDSS and KRFFEY at the read through protein in AT protein of GAV, MAV and PAV strains, the sequence from VDSS to KRFFEY varied among different strains, and the O-glycosylation and phosphorylation sites of A T protein w ere mainly distributed in this region, so it w as deduced that the sequence from VDSSto KRFFEY might be the functional region de- ciding the difference of aphid transmission characteristics betw een strains. The AT protein was mainly located at cell nucleus. The coiled-coils w ere found in the AT proteins of three strains, and the sequences EYEAA and the location of the coiled-coils near the 10 aa were highly conservative. Th